Primary Considerations in Quantitative Mass Spectrometry
Conference paper
First Online:
Abstract
Mass spectrometry is a powerful approach for generating precise, accurate and sensitive quantitative information on the components of a complex sample. This chapter addresses the importance of quantification and the key considerations in the process of developing and performing a quantitative analysis. Rather than providing specific details, the focus here is on introducing the most important considerations associated with developing a quantitative analysis.
Notes
Acknowledgements
The author thanks Dr. Alex Nickel, Biodesix Inc., for helpful discussions during the preparation of this manuscript.
References
- 1.Ramsey MH, Thompson M (2007) Uncertainty from sampling, in the context of fitness for purpose. Accred Qual Assur 12(10):503–513CrossRefGoogle Scholar
- 2.Bethem R, Boison J, Gale PJ, Heller D, Lehotay S, Loo J, Musser S, Price P, Stein S (2003) Establishing the fitness for purpose of mass spectrometric methods. J Am Soc Mass Spectrom 14:528–541CrossRefGoogle Scholar
- 3.Harris DC (2006) Quantitative chemical analysis, 7th edn. Freeman WH, pp 1008Google Scholar
- 4.Green MJ (1996) A practical guide to analytical method validation. Anal Chem 68(9):305A–309ACrossRefGoogle Scholar
- 5.Little TA (2012) Assay development and method validation essentials. BioPharm Int 25(1):p8Google Scholar
- 6.Lee JW, Devanarayan V, Barrett YC et al (2006) Fit-for-purpose method development and validation for successful biomarker measurement. Pharm Res 23:312. doi: 10.1007/s11095-005-9045-3 CrossRefGoogle Scholar
- 7.Tiwari G, Tiwari R (2010) Bioanalytical method validation: an updated review. Pharm Methods 1(1):25–38. doi: 10.4103/2229-4708.72226 CrossRefGoogle Scholar
Copyright information
© Springer Science+Business Media B.V. 2017