Taurine 10 pp 95-106 | Cite as

Neuroprotection by Taurine on HBCD-Induced Apoptosis in PC12 Cells

Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 975)

Abstract

Hexabromocyclododecane (HBCD) is a widely used brominated flame retardant (BFR). Because of their presence in human issues, including brain tissue, concern has been raised on their possible neurotoxicity. Presently, we explored the neuroprotection of taurine against HBCD-induced apoptotic damages in PC12 cells. Cells were pre-treated with taurine before HBCD exposure and the viability was assayed via the methyl-thiazolyl-tetrazolium (MTT) method. Apoptotic features were observed with Hoechst 33342 staining. Apoptotic ratio was measured using flow cytometry with Annexin V-FITC coupled propidium iodide (PI) double staining. The changes in the levels of Bcl-2 and Bax proteins were quantitated by the western blot. The activity of caspase-3 was tested and the results revealed that presence of HBCD decreased cell survival and led to apoptosis in the tested cells. Further, exposure of HBCD reduced protein expression of Bcl-2, increased expression in Bax protein and activity of caspase-3. Taurine attenuated HBCD-induced cell viability loss and cell apoptosis. Moreover, taurine significantly prevented from reducing Bcl-2 protein expression and elevating Bax protein expression and caspase-3 activity induced by HBCD. These results demonstrated that taurine can alleviate HBCD-induced apoptosis by altering Bcl-2 expression and Bax protein and Caspase-3 activity in PC12.

Keywords

HBCD Taurine Apoptosis PC12 cell 

Abbreviations

HBCD

Hexabromocyclododecane

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Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 81102828, 81273037), the natural science foundation of Liaoning province of China (No. 201023054) and the natural science foundation of Shandong province of China (No. 2015ZRB14548).

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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  1. 1.School of Public Health, Dalian Medical UniversityDalianChina
  2. 2.College of Veterinary Medicine, Jilin UniversityChangchunChina
  3. 3.The First Affiliated Hospital of Dalian Medical UniversityDalianChina
  4. 4.School of Pharmaceutical Sciences, Binzhou Medical UniversityYantaiChina

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