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Taurine 10 pp 897-906 | Cite as

Role of Taurine in BDE 209-Induced Oxidative Stress in PC12 Cells

  • Qi Liu
  • Ke Wang
  • Jing Shao
  • Chunna Li
  • Yachen Li
  • Shuangyue Li
  • Xiaohui LiuEmail author
  • Lu HanEmail author
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 975)

Abstract

Polybrominated diphenyl ethers (PBDEs) are globally dispersed throughout the environment, and the levels of some PBDEs in the environment may still be increasing. Previous studies showed that BDE 209 exerted neurodevelopmental and neurobehavioral effects in humans and animals. Oxidative stress is a common mechanism reported in PBDEs-induced neurotoxicity. Taurine, as an antioxidant, whether it is effective in alleviating BDE 209-induced neurotoxicity is still unknown. PC12 cells were exposed to various concentrations of BDE 209 (6.25, 12.5, 25, 50, and 100 μM). 3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay was used to assess the cell viability. 2′,7′-Dichlorofluorescin diacetate (DCFH-DA) detector was used to explore the production of ROS. Acridine orange was used to reflect the permeation of lysosomal membrane. Rhodamine 123 was used to reflect the permeation of mitochondrial membrane. Lactate dehydrogenase and catalase in PC12 cells exposed to BDE 209 were examined by kits. The results showed that taurine could significantly reverse the decreased viability, the serious oxidative stress and abnormal autophagy in PC12 cells exposed to BDE 209. Collectively, our results indicated that taurine could protect PC12 cells from BDE 209-induced neurotoxicity by alleviating oxidative stress.

Keywords

BDE 209 Oxidative stress Taurine PC12 

Abbreviations

CAT

Catalase

LDH

Lactate dehydrogenase

PBDEs

Polybrominated diphenyl ethers

ROS

Reactive oxygen species

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (81302400/H2601 to X.L.; 81273031/H2601 to J.S.); and the startup funding from Dalian Medical University under Talent Introduction Program (201069 to J.S.).

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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  1. 1.Department of Environmental Health and Toxicology, School of Public HealthDalian Medical UniversityDalianChina
  2. 2.Clinical Nutrition DepartmentThe Second Affiliated Hospital of Dalian Medical UniversityDalianChina
  3. 3.The Maternity Affiliated Hospital of Dalian Medical UniversityDalianChina

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