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Biochemical Properties of Urea Transporters

  • Guangping Chen
Chapter
Part of the Subcellular Biochemistry book series (SCBI, volume 73)

Abstract

Urea and urea transporters (UT) are critical to the production of concentrated urine and hence in maintaining body fluid balance. The UT-A1 urea transporter is the major and most important UT isoform in the kidney. Native UT-A1, expressed in the terminal inner medullary collecting duct (IMCD) epithelial cells, is a glycosylated protein with two glycoforms of 117 and 97 kDa. Vasopressin is the major hormone in vivo that rapidly increases urea permeability in the IMCD through increases in phosphorylation and apical plasma-membrane accumulation of UT-A1. The cell signaling pathway for vasopressin-mediated UT-A1 phosphorylation and activity involves two cAMP-dependent signaling pathways: protein kinase A (PKA) and exchange protein activated by cAMP (Epac). In this chapter, we will discuss UT-A1 regulation by phosphorylation, ubiquitination, and glycosylation.

Keywords

Urinary concentration Vasopressin Protein kinase A Phosphorylation Membrane trafficking Ubiquitination Protein degradation N-linked glycosylation 

Notes

Acknowledgments

This work was funded by NIH grants R01-DK087838.

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© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  1. 1.Department of Physiology, and Renal Division Department of MedicineEmory University School of MedicineAtlantaUSA

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