Effects of Rhizobacterial Inoculation of a Preceding Oat Crop on Alfalfa (Medicago sativa L.) Yield

  • Dušica Delić
  • Olivera Stajković-Srbinović
  • Djordje Kuzmanović
  • Nataša Rasulić
  • Srboljub Maksimović
  • Jasmina Radović
  • Aleksandar Simić
Conference paper
First Online:

Abstract

The aim of this study was to test if oats (Avena sativa L.) inoculated with plant growth promoting rhizobacteria (PGPR) could promote growth of alfalfa, Medicago sativa L. as a subsequent crop. A pot experiment was carried out with unsterilized soil under greenhouse conditions. Oat plants were inoculated with strains belonging to Sinorhizobium meliloti, Azotobacter spp., Pseudomonas sp. as well as Enterobacter sp. and Bacillus megaterium. The experiment was conducted as a completely randomized design with eight inoculated treatments and three replicates in each treatment. Inoculated treatments were compared with an uninoculated control treatment. Results indicated that the growth of alfalfa plants was improved by oat inoculation with B. megaterium strain SNj and Azotobacter sp. A1, as well as with a mixture of all strains included in this study. Shoot dry weight of alfalfa was increased by up to 20 % in inoculated treatments compared to the uninoculated control, but the increase varied between strains. The nitrogen content were higher in alfalfa plants exposed to strains B. megaterium SNj (18 %) and Azotobacter sp. A1 (15 %). Results showed that the beneficial effects of inoculation of oat plants on yield and N-assimilation in alfalfa were specific to certain rhizobacteria.

Keywords

Alfalfa Plant growth promotion PGPR Inoculation Preceding crop Oats 
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Notes

Acknowledgement

This research was supported by the Ministry of Science of the Republic of Serbia (grant no. TR 37006).

References

  1. Abbas-Zadeh P, Saleh-Rastin N, Asadi-Rahmani H, Khavazi K, Soltani A, Shoary-Nejati AR, Miransari M (2010) Plant growth-promoting activities of fluorescent pseudomonads, isolated from the Iranian soils. Acta Physiol Plant 32:281–288CrossRefGoogle Scholar
  2. Antoun H, Prevost D (2005) Ecology of plant growth promoting rhizobacteria. In: Siddiqui N (ed) PGPR: biocontrol and biofertilization. Springer, Dordrecht, pp 1–38Google Scholar
  3. Avis TJ, Gravel V, Antoun H, Tweddell RJ (2008) Multifaceted beneficial effects of rhizosphere microorganisms on plant health and productivity. Soil Biol Biochem 40:1733–1740CrossRefGoogle Scholar
  4. Bhattacharyya PN, Jha DK (2012) Plant growth-promoting rhizobacteria (PGPR) emergence in agriculture. World J Microbiol Biotechnol 28:1327–1350PubMedCrossRefGoogle Scholar
  5. Delić D, Stajković-Srbinović O, Kuzmanović Đ, Rasuli N, Maksimović S, Radović J, Simić A (2012) Influence of plant growth promoting rhizobacteria on alfalfa Medicago sativa L. yield by inoculation of a preceding Italian ryegrass, Lolium meltiflorum Lam. In: Barth S, Milbourne D (eds) Breeding strategies for sustainable forage and turf grass improvement. Springer, Dordrecht Heidelberg London New York, pp 333–339Google Scholar
  6. Delić D, Stajković-Srbinović O, Živković S, Protić N, Rasulić N, Kuzmanović Đ, Simić A (2013) Growth promotion of alfalfa, Medicago sativa L by inoculation of a preceding crop with rhizobacteria. Plant Protect 64:35–42Google Scholar
  7. Domit LA, Costa JA, Vidor C, Pereira JS (1990) Inoculation of cereal seeds with Bradyrhizobium japonicum and its effect on soybeans grown in succession. Rev Bras Ciên Solo 14:313–319Google Scholar
  8. Figueiredo MVB, Burity HA, Martinez CR, Chanway CP (2008) Alleviation of drought stress in the common bean (Phaseolus vulgaris L.) by co-inoculation with Paenibacillus polymyxa and Rhizobium tropici. Appl Soil Ecol 40:182–188CrossRefGoogle Scholar
  9. Figueiredo MVB, Seldin L, de Araujo FF, Mariano RLR (2010) Plant growth promoting rhizobacteria: fundamentals and applications. In: Maheshwari DK (ed) Plant growth and health promoting bacteria, Microbiology monographs 18. Springer, Berlin/Heidelberg, pp 22–36Google Scholar
  10. Franche C, Lindström K, Elmerich C (2009) Nitrogen-fixing bacteria associated with leguminous and non-leguminous plants. Plant and Soil 321:35–59CrossRefGoogle Scholar
  11. Goos RJ, Johnson BE, Carr PM (2001) Establishment of Bradyrhizobium japonicum for soybean by inoculation of a preceding wheat crop. Plant Soil 235:127–133CrossRefGoogle Scholar
  12. Gray EJ, Smith DL (2005) Intracellular and extracellular PGPR: commonalities and distinctions in the plant-bacterium signaling processes. Soil Biol Biochem 37:395–412CrossRefGoogle Scholar
  13. Gupta CP, Dubey RC, Maheshwari DK (2002) Plant growth enhancement and suppression of Macrophomina phaseolina causing charcoal rot of peanut by fluorescent Pseudomonas. Biol Fertil Soils 35:399–405CrossRefGoogle Scholar
  14. Hayat R, Ali S (2010) Nitrogen fixation of legumes and yield of wheat under legumes-wheat rotation in Pothwar. Pak J Bot 42(4):2317–2326Google Scholar
  15. Holt JG (1984–1989) Bergey’s manual of systematic bacteriology, vol 1–4. Williams and Wilkins, BaltimoreGoogle Scholar
  16. King EO, Ward MK, Raney DE (1954) Two simple media for the demonstration of pyocanin and fluorescin. J Lab Clin Med 44:301–307PubMedGoogle Scholar
  17. Kumar A, Prakash A, Johri BN (2011) Bacillus as PGPR in crop ecosystem. In: Maheshwari DK (ed) Bacteria in Agrobiology: Crop Ecosystems. Springer, Berlin/Heidelberg, pp 37–59CrossRefGoogle Scholar
  18. Lassiter RB, Jordan LJ, Wilkerson GG, Shew BB, Brandenburg LR (2011) Influence of cover crops on weed management in strip tillage peanut. Weed Technol 25(4):568–573CrossRefGoogle Scholar
  19. Lucy M, Reed E, Glick BR (2004) Applications of free living plant growth-promoting rhizobacteria. Review. Antonie Van Leeuwenhoek 86:1–25PubMedCrossRefGoogle Scholar
  20. Martínez-Viveros O, Jorquera MA, Crowley DE, Gajardo G, Mora ML (2010) Mechanisms and practical considerations involved in plant growth promotion by rhizobacteria. J Soil Sci Plant Nutr 10:293–319CrossRefGoogle Scholar
  21. Mehboob I, Zahir ZA, Mahboob A, Shahzad SM, Jawad A, Arshad M (2008) Preliminary screening of Rhizobium isolates for improving growth of maize seedlings under axenic conditions. Soil Environ 27:64–71Google Scholar
  22. Minorsky PV (2008) On the inside. Plant Physiol 146:323–324CrossRefPubMedCentralGoogle Scholar
  23. Nagabhushana GG, Worsham AD, Yenish JP (2001) Allelopathic cover crops to reduce herbicide use in sustainable agriculture systems. Allelopathy J 8:133–146Google Scholar
  24. Niranjan Raj S, Shetty HS, Reddy MS (2005) Plant growth-promoting rhizobacteria: potential green alternative for plant productivity. In: Siddiqui ZA (ed) PGPR: biocontrol and biofertilization. Springer, Dordrecht, pp 197–216Google Scholar
  25. Reeves DW, Price AJ, Patterson MG (2005) Evaluation of three winter cereals for weed control in conservation-tillage non transgenic cotton. Weed Technol 19:731–736CrossRefGoogle Scholar
  26. Richardson AE, Barea JM, McNeill AM, Prigent-Combaret C (2009) Acquisition of phosphorus and nitrogen in the rhizosphere and plant growth promotion by microorganisms. Plant Soil 321:305–339CrossRefGoogle Scholar
  27. Sarić Z (1989) Praktikum iz mikrobiologije. Naučna knjiga, Beograd, pp 1–193Google Scholar
  28. Tchan Y-T, New PB (1984) Genus I. Azotobacter Beijerinck 1901. In: Holt JG, Krieg NR (eds) Bergey’s manual of systematic bacteriology, vol 1. Williams and Wilkins, Baltimore, pp 220–229Google Scholar
  29. Vessey JK (2003) Plant growth promoting rhizobacteria as biofertilizers. Plant Soil 255:571–586CrossRefGoogle Scholar
  30. Vincent MJ (1970) Manual for the practical study of root-nodule bacteria. In: IBP handbook, vol 15. Blackwell, Oxford, pp 3–4Google Scholar
  31. Zabihi HR, Savaghebi GR, Khavazi K, Ganjali A, Miransari M (2010) Pseudomonas bacteria and phosphorous fertilization, affecting wheat (Triticum aestivum L.) yield and P uptake under greenhouse and field conditions. Acta Physiol Plantarum 33(1):145–152CrossRefGoogle Scholar
  32. Zhang F, Dasthi N, Hynes RK, Smith DL (1996) Plant growth promoting rhizobacteria and Soybean [Glycine max (L.) Merr.] Nodulation and nitrogen fixation at suboptimal root zone temperatures. Ann Bot 77:453–459CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Dušica Delić
    • 1
  • Olivera Stajković-Srbinović
    • 1
  • Djordje Kuzmanović
    • 1
  • Nataša Rasulić
    • 1
  • Srboljub Maksimović
    • 2
  • Jasmina Radović
    • 3
  • Aleksandar Simić
    • 4
  1. 1.Department of MicrobiologyInstitute of Soil ScienceBelgradeSerbia
  2. 2.Department of Agro-chemistryInstitute of Soil ScienceBelgradeSerbia
  3. 3.Institute for Forage CropsGloboder, KruševacSerbia
  4. 4.Faculty of AgricultureUniversity of BelgradeBelgradeSerbia

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