Are Chlorophyll-Carotenoid Interactions Responsible for Rapidly Reversible Non-Photochemical Fluorescence Quenching?

Chapter
Part of the Advances in Photosynthesis and Respiration book series (AIPH, volume 40)

Summary

Photoprotective thermal energy dissipation (as assessed via non-photochemical quenching of singlet-excited chlorophyll a, NPQ) in plants is driven by various mechanisms occurring over different time scales. The rapid and reversible part of NPQ, also called qE (for energy-dependent quenching), was demonstrated to correlate with the twisting of a neoxanthin molecule in the light-harvesting antenna as observed by resonance Raman spectroscopy (Nature 450: 575–578, 2007). Interestingly, the extent of fluorescence quenching correlates with the change in Raman signal in different situations: during NPQ in vivo, during fluorescence quenching upon aggregation of LHCII (the major light-harvesting complex in plants), and in crystals of LHCII. In the same study, it was proposed that the quenching is caused by excitation energy transfer from chlorophyll a to lutein in LHCII after a structural change that correlates with the twisting of the neoxanthin. However, this view has been challenged by others for different reasons. Here we discuss the arguments in favor and against this mechanism. A short overview is given of the spectroscopic data on chlorophyll-carotenoid interactions in plant light-harvesting systems, the changes in interactions upon aggregation or crystallization, and the possible relationship to the mechanism of NPQ.

Abbreviations:

B4C

Band 4 complex;

C2

Dimeric core of PS II;

C2S2M2

Supercomplex of PS II consisting of a dimeric core surrounded by the outer light-harvesting complexes (LHCs): 4 major (LHCII) and 6 minor ones (two each of CP24, CP26 and CP29);

Car

Carotenoid;

Chl

Chlorophyll;

Chl 610

Chl A1;

Chl 612

Chl A2;

CP24, CP26, CP29

Minor light-harvesting complexes of photosystem II;

CT

Charge-transfer;

DES

De-epoxidation state;

EET

Excitation energy transfer;

LHC

Light-harvesting complex;

LHCII

Light-harvesting complex II;

LHCII-M

Moderately coupled LHCII trimer;

LHCII-S

Strongly coupled LHCII trimer;

Lut1

Lutein 1;

N

Neoxanthin;

NPQ

Non-photochemical quenching of chlorophyll fluorescence;

PS I

Photosystem I;

PS II

Photosystem II;

qE

Energy-dependent quenching;

RC

Reaction center;

ROS

Reactive oxygen species;

V

Violaxanthin;

Z

Zeaxanthin

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  1. 1.Laboratory of BiophysicsWageningen UniversityWageningenThe Netherlands

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