High Throughput Screening of Allergy

Chapter
First Online:
Part of the Translational Bioinformatics book series (TRBIO, volume 8)

Abstract

Allergen avoidance and specific immunotherapy that are based on an accurate diagnosis of allergy have proven to be helpful for the prevention and treatment of allergic diseases. Detection of specific IgE against different allergens in serum or plasma can be used as an important criterion for allergy diagnosis. Conventional methods for the detection of IgE in the clinic are mainly enzyme-linked immunosorbent analysis (ELISA), fluorescence enzyme immunoassays, and radioimmunoassays (RIAs). However, it is difficult to achieve high throughput screening by these conventional methods. Microarrays can test more than 1000 known allergens and are able to resolve reactivities to various allergen components, which is necessary for clinical diagnosis and treatment. Thus, allergen microarrays provide a versatile platform for detecting specific IgE or other types of antibodies against thousands of allergens in a parallel and high throughput way and are currently used in diagnosis of allergy. In this chapter we will provide an overview of allergen microarrays.

Keywords

Allergen microarrays High throughput screening Allergy diagnosis 
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References

  1. Angenendt P. Progress in protein and antibody microarray technology. Drug Discovery Today. 2005;10(7):503–11.CrossRefPubMedGoogle Scholar
  2. Asturias JA, Ibarrola I, Bartolome B, Ojeda I, Malet A, Martinez A. Purification and characterization of Pla a 1, a major allergen from Platanus acerifolia pollen. Allergy. 2002;57(3):221–7.CrossRefPubMedGoogle Scholar
  3. Cabauatan CR, Lupinek C, Scheiblhofer S, Weiss R, Focke-Tejkl M, Bhalla PL, Singh MB, Knight PA, van Hage M, Ramos JD, et al. Allergen microarray detects high prevalence of asymptomatic IgE sensitizations to tropical pollen-derived carbohydrates. J Allergy Clin Immunol. 2014;133(3):910–914e5.CrossRefPubMedGoogle Scholar
  4. Canonica GW, Ansotegui IJ, Pawankar R, Schmid-Grendelmeier P, van Hage M, Baena-Cagnani CE, Melioli G, Nunes C, Passalacqua G, Rosenwasser L, et al. A WAO—ARIA—GA(2)LEN consensus document on molecular-based allergy diagnostics. World Allergy Organ J. 2013;6(1):17.CrossRefPubMedPubMedCentralGoogle Scholar
  5. Demoly P, Adkinson NF, Brockow K, Castells M, Chiriac AM, Greenberger PA, Khan DA, Lang DM, Park HS, Pichler W, et al. International consensus on drug allergy. Allergy. 2014;69(4):420–37.CrossRefPubMedGoogle Scholar
  6. Ebo DG, Hagendorens MM, De Knop KJ, Verweij MM, Bridts CH, De Clerck LS, Stevens WJ. Component-resolved diagnosis from latex allergy by microarray. Clin Exp Allergy: J Br Soc Allergy Clin Immunol. 2010;40(2):348–58.CrossRefGoogle Scholar
  7. Ebo DG, Leysen J, Mayorga C, Rozieres A, Knol EF, Terreehorst I. The in vitro diagnosis of drug allergy: status and perspectives. Allergy. 2011;66(10):1275–86.CrossRefPubMedGoogle Scholar
  8. Grier TJ. Laboratory methods for allergen extract analysis and quality control. Clin Rev Allergy Immunol. 2001;21(2–3):111–40.CrossRefPubMedGoogle Scholar
  9. Hamilton RG. Clinical laboratory assessment of immediate-type hypersensitivity. J Allergy Clin Immunol. 2010;125(2):S284–96.CrossRefPubMedGoogle Scholar
  10. Harwanegg C, Hiller R. Protein microarrays in diagnosing IgE-mediated diseases: spotting allergy at the molecular level. Expert Rev Mol Diagn. 2004;4(4):539–48.CrossRefPubMedGoogle Scholar
  11. Jackson AM, Boutell J, Cooley N, He M. Cell-free protein synthesis for proteomics. Briefings Funct Genomics Proteomics. 2004;2(4):308–19.CrossRefGoogle Scholar
  12. Jeong KY, Hong CS, Lee JS, Park JW. Optimization of allergen standardization. Yonsei Med J. 2011;52(3):393–400.CrossRefPubMedPubMedCentralGoogle Scholar
  13. Kamemura N, Tada H, Shimojo N, Morita Y, Kohno Y, Ichioka T, Suzuki K, Kubota K, Hiyoshi M, Kido H. Intrauterine sensitization of allergen-specific IgE analyzed by a highly sensitive new allergen microarray. J Allergy Clin Immunol. 2012;130(1):113–121e2.CrossRefPubMedGoogle Scholar
  14. Kumble KD. An update on using protein microarrays in drug discovery. Expert Opin Drug Discov. 2007;2(11):1467–76.CrossRefPubMedGoogle Scholar
  15. Kusnezow W, Jacob A, Walijew A, Diehl F, Hoheisel JD. Antibody microarrays: an evaluation of production parameters. Proteomics. 2003;3(3):254–64.CrossRefPubMedGoogle Scholar
  16. Larsen JN, Dreborg S. Standardization of allergen extracts. Methods Mol Med. 2008;138:133–45.CrossRefPubMedGoogle Scholar
  17. Lin J, Bruni FM, Fu Z, Maloney J, Bardina L, Boner AL, Gimenez G, Sampson HA. A bioinformatics approach to identify patients with symptomatic peanut allergy using peptide microarray immunoassay. J Allergy Clin Immunol. 2012;129(5):1321–1328e5.CrossRefPubMedPubMedCentralGoogle Scholar
  18. Lin J, Renault N, Haas H, Schramm G, Vieths S, Vogel L, Falcone FH, Alcocer MJ. A novel tool for the detection of allergic sensitization combining protein microarrays with human basophils. Clin Exp Allergy: J Br Soc Allergy Clin Immunol. 2007;37(12):1854–62.CrossRefGoogle Scholar
  19. Maloney JM, Rudengren M, Ahlstedt S, Bock SA, Sampson HA. The use of serum-specific IgE measurements for the diagnosis of peanut, tree nut, and seed allergy. J Allergy Clin Immunol. 2008;122(1):145–51.CrossRefPubMedGoogle Scholar
  20. Manfredi M, Severino M, Testi S, Macchia D, Ermini G, Pichler WJ, Campi P. Detection of specific IgE to quinolones. J Allergy Clin Immunol. 2004;113(1):155–60.CrossRefPubMedGoogle Scholar
  21. Mittag D, Akkerdaas J, Ballmer-Weber BK, Vogel L, Wensing M, Becker WM, Koppelman SJ, Knulst AC, Helbling A, Hefle SL, et al. Ara h 8, a Bet v 1-homologous allergen from peanut, is a major allergen in patients with combined birch pollen and peanut allergy. J Allergy Clin Immunol. 2004;114(6):1410–7.CrossRefPubMedGoogle Scholar
  22. Ott H, Baron JM, Heise R, Ocklenburg C, Stanzel S, Merk HF, Niggemann B, Beyer K. Clinical usefulness of microarray-based IgE detection in children with suspected food allergy. Allergy. 2008;63(11):1521–8.CrossRefPubMedGoogle Scholar
  23. Pfiffner P, Stadler BM, Rasi C, Scala E, Mari A. Cross-reactions vs co-sensitization evaluated by in silico motifs and in vitro IgE microarray testing. Allergy. 2012;67(2):210–6.CrossRefPubMedGoogle Scholar
  24. Pichler WJ. Delayed drug hypersensitivity reactions. Ann Intern Med. 2003;139(8):683–93.CrossRefPubMedGoogle Scholar
  25. Predki PF, Mattoon D, Bangham R, Schweitzer B, Michaud G. Protein microarrays: a new tool for profiling antibody cross-reactivity. Hum Antibodies. 2005;14(1–2):7–15.PubMedGoogle Scholar
  26. Riecken S, Lindner B, Petersen A, Jappe U, Becker WM. Purification and characterization of natural Ara h 8, the Bet v 1 homologous allergen from peanut, provides a novel isoform. Biol Chem. 2008;389(4):415–23.CrossRefPubMedGoogle Scholar
  27. Romano A, Demoly P. Recent advances in the diagnosis of drug allergy. Curr Opin Allergy Clin Immunol. 2007;7(4):299–303.CrossRefPubMedGoogle Scholar
  28. Romano A, Gueant-Rodriguez RM, Viola M, Amoghly F, Gaeta F, Nicolas JP, Gueant JL. Diagnosing immediate reactions to cephalosporins. Clin Exp Allergy: J Br Soc Allergy Clin Immunol. 2005;35(9):1234–42.CrossRefGoogle Scholar
  29. Shreffler WG. Microarrayed recombinant allergens for diagnostic testing. J Allergy Clin Immunol. 2011;127(4):843–9 quiz 50-1.CrossRefPubMedGoogle Scholar
  30. Smith AH, Vrtis JM, Kodadek T. The potential of protein-detecting microarrays for clinical diagnostics. Adv Clin Chem. 2004;38:217–38.CrossRefPubMedGoogle Scholar
  31. Soares-Weiser K, Takwoingi Y, Panesar SS, Muraro A, Werfel T, Hoffmann-Sommergruber K, Roberts G, Halken S, Poulsen L, van Ree R, et al. The diagnosis of food allergy: a systematic review and meta-analysis. Allergy. 2014;69(1):76–86.CrossRefPubMedGoogle Scholar
  32. Spisak S, Guttman A. Biomedical applications of protein microarrays. Curr Med Chem. 2009;16(22):2806–15.CrossRefPubMedGoogle Scholar
  33. Stoevesandt O, Taussig MJ, He M. Producing protein microarrays from DNA microarrays. Methods Mol Biol. 2011;785:265–76.CrossRefPubMedGoogle Scholar
  34. Sturm GJ, Kranzelbinder B, Sturm EM, Heinemann A, Groselj-Strele A, Aberer W. The basophil activation test in the diagnosis of allergy: technical issues and critical factors. Allergy. 2009;64(9):1319–26.CrossRefPubMedGoogle Scholar
  35. Suzuki K, Hiyoshi M, Tada H, Bando M, Ichioka T, Kamemura N, Kido H. Allergen diagnosis microarray with high-density immobilization capacity using diamond-like carbon-coated chips for profiling allergen-specific IgE and other immunoglobulins. Anal Chim Acta. 2011;706(2):321–7.CrossRefPubMedGoogle Scholar
  36. Templin MF, Stoll D, Schwenk JM, Potz O, Kramer S, Joos TO. Protein microarrays: promising tools for proteomic research. Proteomics. 2003;3(11):2155–66.CrossRefPubMedGoogle Scholar
  37. Treudler R, Simon JC. Overview of component resolved diagnostics. Curr Allergy Asthma Rep. 2013;13(1):110–7.CrossRefPubMedGoogle Scholar
  38. Wang RQ, Zhang HY. Two hundred thousands results of allergen specific IgE detection. Chin J Allergy Clin Immunol. 2012;6(1):18–23.Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  1. 1.Guangdong Provincial Key Laboratory of Allergy and Clinical Immunology, The State Key Clinical Specialty in Allergy, The State Key Laboratory of Respiratory DiseaseThe Second Affiliated Hospital of Guangzhou Medical UniversityGuangzhouChina

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