The piRNA Pathway Guards the Germline Genome Against Transposable Elements

  • Katalin Fejes Tóth
  • Dubravka Pezic
  • Evelyn Stuwe
  • Alexandre Webster
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 886)

Abstract

Transposable elements (TEs) have the capacity to replicate and insert into new genomic locations. This contributs significantly to evolution of genomes, but can also result in DNA breaks and illegitimate recombination, and therefore poses a significant threat to genomic integrity. Excess damage to the germ cell genome results in sterility. A specific RNA silencing pathway, termed the piRNA pathway operates in germ cells of animals to control TE activity. At the core of the piRNA pathway is a ribonucleoprotein complex consisting of a small RNA, called piRNA, and a protein from the PIWI subfamily of Argonaute nucleases. The piRNA pathway relies on the specificity provided by the piRNA sequence to recognize complementary TE targets, while effector functions are provided by the PIWI protein. PIWI-piRNA complexes silence TEs both at the transcriptional level – by attracting repressive chromatin modifications to genomic targets – and at the posttranscriptional level – by cleaving TE transcripts in the cytoplasm. Impairment of the piRNA pathway leads to overexpression of TEs, significantly compromised genome structure and, invariably, germ cell death and sterility.

The piRNA pathway is best understood in the fruit fly, Drosophila melanogaster, and in mouse. This Chapter gives an overview of current knowledge on piRNA biogenesis, and mechanistic details of both transcriptional and posttranscriptional TE silencing by the piRNA pathway. It further focuses on the importance of post-translational modifications and subcellular localization of the piRNA machinery. Finally, it provides a brief description of analogous pathways in other systems.

Keywords

piRNA Small RNA Argonautes Piwi proteins TE Transposon Transposable element Tudor domain Transcriptional silencing Posttranscriptional silencing Heterochromatin H3K9me3 DNA methylation Ping-Pong cycle Germ granules Nuage Pole plasm Pi-bodies Intramitochondrial cement 

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Katalin Fejes Tóth
    • 1
  • Dubravka Pezic
    • 1
  • Evelyn Stuwe
    • 1
  • Alexandre Webster
    • 1
  1. 1.Division of Biology and BioengineeringCalifornia Institute of TechnologyPasadenaUSA

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