Effects of Protein Phosphorylation on the Properties of Thylakoid Membranes
Part of the
Advances in Agricultural Biotechnology
book series (AABI, volume 3)
The State 1 to State 2 transition in higher plant chloroplasts involves the reversible phosphorylation of thylakoid membrane proteins (Horton 1983). This phosphorylation can alter the distribution of energy between PSII and PSI by either, or both, an alteration in the absorption cross-section of PSI (α) or by a change in spillover. An indication of mechanism is given by room temperature fluorescence induction curves of phosphorylated and non-phosphorylated thylakoids in the presence of DCMU (Fig.1). A lowering of fluorescence such that the Fv/Fm ratio is unchanged indicates a decrease in α whereas a preferential quenching of Fv (i.e. a decrease in the Fv/Fm ratio) indicates an increase in spillover of energy from PSII to PSI. We have previously shown (Horton, Black 1983) that the predominance of either factor depends upon the ionic composition of the suspension medium with phosphorylation promoting an increase in spillover only at low Mg2+ concentrations.
KeywordsThylakoid Membrane Methyl Viologen Continuous Light Reversible Phosphorylation Fluorescence Induction Curve
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Horton, P (1983). Control of chloroplast electron transport by phosphorylation of thylakoid proteins. FEBS Letters 152, 47–51CrossRefGoogle Scholar
Horton, P and Black, MT (1983). A comparison between cation and protein phosphorylation effects on the fluorescence induction curve in chloroplasts treated with DCMU. Biochim. Biophys. Acta, 722, 214–218.CrossRefGoogle Scholar
Argyroudi-Akoyunoglou, JH and Akoyunoglou G (1970). Photoinduced changes in the chlorophyll a
to chlorophyll b
ratio in young bean plants. Plant Physiol. 46, 247–249.PubMedCrossRefGoogle Scholar
Armond, PA; Arntzen, CJ; Briantis, J.M and Vernotte C (1976) Differentiation of chloroplast lamellae. Light harvesting efficiency and granal development. Arch.Biochem.Biophys. 175, 54–63.PubMedCrossRefGoogle Scholar
Anderson, JM (1980). P-700 content and polypeptide profile of chlorophyll-protein complexes of spinach and barley thylakoids. Biochim. Biophys. Acta, 591, 113–126.PubMedCrossRefGoogle Scholar
Kyle, DJ; Staehelin, LA and Arntzen, CJ (1983). Lateral mobility of the light harvesting complex in chloroplast membranes controls excitation distribution in higher plants. Arch. Biochem. Biophys. 222, 527–541.PubMedCrossRefGoogle Scholar
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