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Effects of Protein Phosphorylation on the Properties of Thylakoid Membranes

  • Michael Black
  • Peter Horton
  • Christine Foyer
Part of the Advances in Agricultural Biotechnology book series (AABI, volume 3)

Abstract

The State 1 to State 2 transition in higher plant chloroplasts involves the reversible phosphorylation of thylakoid membrane proteins (Horton 1983). This phosphorylation can alter the distribution of energy between PSII and PSI by either, or both, an alteration in the absorption cross-section of PSI (α) or by a change in spillover. An indication of mechanism is given by room temperature fluorescence induction curves of phosphorylated and non-phosphorylated thylakoids in the presence of DCMU (Fig.1). A lowering of fluorescence such that the Fv/Fm ratio is unchanged indicates a decrease in α whereas a preferential quenching of Fv (i.e. a decrease in the Fv/Fm ratio) indicates an increase in spillover of energy from PSII to PSI. We have previously shown (Horton, Black 1983) that the predominance of either factor depends upon the ionic composition of the suspension medium with phosphorylation promoting an increase in spillover only at low Mg2+ concentrations.

Keywords

Thylakoid Membrane Methyl Viologen Continuous Light Reversible Phosphorylation Fluorescence Induction Curve 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer Science+Business Media Dordrecht 1984

Authors and Affiliations

  • Michael Black
    • 1
  • Peter Horton
    • 1
  • Christine Foyer
    • 2
  1. 1.Department of Biochemistry and ARC Research Group on PhotosynthesisUniversity of SheffieldSheffieldUK
  2. 2.ARC Research Group on Photosynthesis, Department of BotanyUniversity of SheffieldSheffieldUK

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