In vitro induction of haploid plants from the gametophytes of lily and tulip

  • R. W. Van Den Bulk
  • J. M. Van Tuyl
Chapter
Part of the Current Plant Science and Biotechnology in Agriculture book series (PSBA, volume 29)

Abstract

As a result of sporophytic cell division and proliferation of the male or female gametophytes in culture, embryos or calli may develop from which haploid plantlets may be regenerated. The first haploid plant from the in vitro culture of anthers was verified in 1964 for Datura innoxia (Guha & Maheshwari, 1964), whereas the first haploid plant from unpollinated ovaries was obtained in 1976 for Hordeum vulgare (San Noeum, 1976). The induction of haploid plants from male or female gamethophytes by in vitro culture is now possible for many species (Yang & Zhou, 1990; Sangwan & SangwanNorreel, 1990). In vitro culture of anthers has been the most efficient method for regenerating (doubled) haploid plants, especially for brassicaceous, graminaceous, or solanaceous species. In the last decade, microspore culture has become an important technique to obtain haploid or doubled haploid plants. The culture of isolated microspores has several advantages over culture of whole anthers. Regenerated plants will undoubtedly have been derived from the microspores and not from anther tissue. Also, microspores can be isolated in large numbers and are promising targets for in vitro manipulation and ontogenetic studies. Moreover, for some crops, e.g., Hordeum vulgare (Hoekstra et al.,1992) and Brassica napus (Siebel & Pauls, 1989), a higher efficiency of haploid production can be reached with microspore culture. When anther or microspore culture cannot be applied, e.g., when microspores are not formed due to male-sterility, or when androgenesis is hampered by low response or albinism of regenerated plants, culture of unpollinated ovaries or ovules may be an alternative to haploid derivation (Yang & Zhou, 1982).

Keywords

Anther Culture Microspore Culture Haploid Plant Fusaric Acid Ovary Culture 
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References

  1. Bennici, A., 1979. Cytological chimeras in plants regenerated from Lilium longiflorum tissues grown in vitro. Z. Pflanzenzüchtg. 82: 349–353.Google Scholar
  2. Custers, J.B.M., W. Eikelboom, J.H.W. Bergervoet & J.P. van Eijk, 1992. In ovulo embryo culture of tulip (Tulipa L.); effects of culture conditions on seedling and bulblet formation. Scientia Hort. 51: 111–122.CrossRefGoogle Scholar
  3. Duijs, J.G., R.E. Voorrips, D.L. Visser & J.B.M. Custers, 1992. Microspore culture is successful in most crop types of Brassica oleracea L. Euphytica 60: 45–55.Google Scholar
  4. Gaillard, A., P. Vergne & M. Beckert, 1991. Optimization of maize microspore isolation and culture conditions for reliable plant regeneration. Plant Cell Rep. 10: 55–58.CrossRefGoogle Scholar
  5. Gu, Z.-P. & K.-C. Cheng, 1982. Studies on induction of pollen plantlets from the anther cultures of lily. Acta Bot. Sin. 24: 28–32.Google Scholar
  6. Gu, Z.-P. & K.-C. Cheng, 1983. In vitro induction of haploid plantlets from unpollinated young ovaries of lily and its embryological observations. Acta Bot. Sin. 25: 24–30.Google Scholar
  7. Guha, S. & S.C. Maheshwari, 1964. In vitro production of embryos from anthers of Datura. Nature 204: 497.Google Scholar
  8. Hassawi D.S. & G.H Liang, 1991. Antimitotic agents: effects of double haploid production in wheat. Crop Sci. 31: 723–726.CrossRefGoogle Scholar
  9. Hoekstra, S., M.H. van Zijderveld, J.D. Louwerse, F. Heidekamp & F. van der Mark, 1992.Google Scholar
  10. Anther and microspore culture of Hordeum vulgare L. cv. Igri. Plant Sci. 86: 89–96. Idzikowska, K. & F. Mlodzianowski, 1979. Cell wall formation in multinucleate pollen grainsGoogle Scholar
  11. of Hordeum vulgare anthers cultures in vitro. Acta Soc. Bot. Pol. 48: 377–380.Google Scholar
  12. Lichter, R., 1982. Induction of haploid plants from isolated pollen of Brassica napus. Z.Google Scholar
  13. Pflanzenphysiol. 105: 427–434.Google Scholar
  14. Lichter, R., 1989. Efficient yield of embryoids by culture of isolated microspores of different Brassicaceae species. Plant Breed. 103: 119–123.CrossRefGoogle Scholar
  15. Löffler, H.J.M., J.R. Mouris & M.J. van Harmelen, 1990. In vitro selection for resistance against Fusarium oxysporum in lily: prospects. The Lily Yearbook of the North Am. Lily Soc. 43: 56–60.Google Scholar
  16. Melchinger, A.E., 1990. Use of molecular markers in breeding for oligogenic disease resistance. Plant Breed. 104: 1–19.CrossRefGoogle Scholar
  17. Murashige, T. & F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497.Google Scholar
  18. Prakash, J. & K.L. Giles, 1986. Production of doubled haploids in oriental lilies. In: W. Horn, C.J. Jensen, W. Odenbach & O. Schieder (Eds.), Genetic Manipulation in Plant Breeding, pp. 335–337. Walter de Gruyter, Berlin.Google Scholar
  19. Qu, Y., M.C. Mok, D.W.S. Mok & J.R. Stang, 1988. Phenotypic and cytological variation among plants derived from anther cultures of Lilium longiflorum. In Vitro Cellular and Devel. Biol. 24: 471–476.Google Scholar
  20. Sangwan, R.S. & B.S. Sangwan-Norreel, 1990. Anther and pollen culture. In: S.S. Bhojwani (Ed.), Developments in Crop Science 19. Plant Tissue Culture: Applications and Limitations, pp. 220–241. Elsevier, Amsterdam.CrossRefGoogle Scholar
  21. San Noeum, L.H., 1976. Haploïdes d’Hordeum vulgare L. par culture in vitro non fécondés. Ann. Amélior. Plantes 26: 751–754.Google Scholar
  22. Sharp, W.R., R.S. Raskin & H.E. Sommer, 1971. Haploidy in Lilium. Phytomorphology 21: 334–337.Google Scholar
  23. Siebel, J. & K.P. Pauls, 1989. A comparison of anther and microspore culture as a breeding tool in Brassica napus. Theor. Appl. Genet. 78: 473–479.Google Scholar
  24. Tanaka, I. & M. Ito, 1980. Induction of typical cell division in isolated microspores of Lilium longiflorum and Tulipa gesneriana. Plant Sci. Lett. 17: 279–285.Google Scholar
  25. Tanaka, I. & M. Ito, 1981a. Control of division patterns in explanted microspores of Tulipa gesneriana. Protoplasma 108: 329–340.CrossRefGoogle Scholar
  26. Tanaka, I. & M. Ito, 1981b. Studies on microspore development in liliaceous plants III. Pollen tube development in lily pollen cultured from the uninucleate microspore stage. Plant Cell Physiol. 22: 149–153.Google Scholar
  27. Tanaka, I. & M. Ito, 1982. Additional mitoses induced by bromodeoxyuridine in explanted tulip microspores. Plant Sci. Lett. 27: 37–42.Google Scholar
  28. Tanaka, I., C. Kitazume & M. Ito, 1987. The isolation and culture of lily pollen protoplasts. Plant Sci. 50: 205–211.CrossRefGoogle Scholar
  29. Tanaka, I., T. Taguchi & M. Ito, 1979. Studies on microspore development in liliaceous plants.Google Scholar
  30. I. The duration of the cell cycle and developmental aspects in lily microspores. Bot. Mag. Tokyo 92: 291–298.Google Scholar
  31. Tanaka, I., T. Taguchi & M. Ito, 1980. Studies on microspore development in liliaceous plants.Google Scholar
  32. II. The behavior of explanted microspores of the lily, Lilium longiflorum. Plant Cell Physiol. 21: 667–676.Google Scholar
  33. Ueda, K., Y. Miyamoto & I. Tanaka, 1990. Fusion studies of pollen protoplasts and generative cell protoplasts in Lilium longiflorum. Plant Sci. 72: 259–266.CrossRefGoogle Scholar
  34. Van den Bulk, R.W., H.P.J. de Vries-van Hulten & J.J.M. Dons, 1992. Formation of multinucleate lily microspores in culture. Acta Hort. 325: 649–654.Google Scholar
  35. Van Eijk, J.P., L.W.D. van Raamsdonk, W. Eikelboom & R.J. Bino, 1991. Interspecific crosses between Tulipa gesneriana cultivars and wild Tulipa species - a survey. Sex. Plant Rep. 4: 1–5.Google Scholar
  36. Van Tuyl, J.M., H. Meijer & M.P. van Diën, 1992. The use of oryzalin as an alternative for colchicine in in-vitro chromosome doubling of Lilium and Nerine. Acta Hort. 325: 625–630.Google Scholar
  37. Van Tuyl, J.M., M.C.M. van Creij, W. Eikelboom, D.M.F.J Kerckhoffs & B. Meijer, 1993. New genetic variation in the Lilium and Tulipa assortment by wide hybridization. In: T. Schiva & A. Mercuri (Eds.), Proceedings XVIIth Eucarpia Symposium, pp. 141–149. Istituto sperimentale per la Floricoltura, Sanremo.Google Scholar
  38. Yang, H.Y. & C. Zhou, 1982. In vitro induction of haploid plants from unpollinated ovaries and ovules. Theor. Appl. Genet. 63: 97–104.Google Scholar
  39. Yang, H.Y. & C. Zhou, 1990. In vitro gynogenesis. In: S.S. Bhojwani (Ed.), Developments in Crop Science 19. Plant Tissue Culture: Applications and Limitations, pp. 242–258. Elsevier, Amsterdam.CrossRefGoogle Scholar
  40. Zhou, C. & Y. Wu, 1990. Two pathways in pollen protoplast culture: cell divisions and tube growth. In: H.J.J. Nijkamp, L.H.W. van der Plas & J. van Aartrijk (Eds.), Progress in Plant Cellular and Molecular Biology, pp. 222–227. Kluwer Academic Publishers, Dordrecht.CrossRefGoogle Scholar

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© Springer Science+Business Media Dordrecht 1997

Authors and Affiliations

  • R. W. Van Den Bulk
  • J. M. Van Tuyl

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