Animal Cell Technology: Basic & Applied Aspects pp 395-399 | Cite as
Mechanisms of Invasion Inhibition of Hepatoma Cells by Tea Components and Sera from Tea-Fed Rats
Abstract
We previously confirmed that higher concentrations of tea extracts and catechins suppress the proliferation and invasion of a rat ascites hepatoma cell line, AH109A [12] and also induce apoptosis and cell cycle arrest in AH109A cells [3]. However, whether or not drinking tea each day really has certain preventive and therapeutic effects against the invasion of tumor cells is unclear. The mechanisms of the inhibition of invasion of cancer cells by the sera from tea-or EGCG-treated rats remain unknown. To answer these questions, in this study we employed ex vivo models of the invasion of rat hepatoma cells across the rat mesothelial cell (M-cell) monolayer in the co-culture, and systematically investigated the effects and mechanisms of action of sera from low concentration tea-and EGCG-treated rats against the invasion of rat hepatoma cells.
Keywords
Hepatoma Cell Minimum Essential Medium Oral Intubation AH109A Cell Ethylendiaminetetraacetic AcidPreview
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References
- 1.Miura, Y., Shiomi, H., Sakai, F., and Yagasaki, K.: Assay system for screening food components that have anti-proliferative and anti-invasive activity to rat ascites hepatoma cells: In vitro and ex vivo effects of green tea extract. Cytotechnology 23 (1997), 127–132.PubMedCrossRefGoogle Scholar
- 2.Zhang, G., Miura, Y., and Yagasaki, K.: Effects of green, oolong and black teas and related components on the proliferation and invasion of hepatoma cells in culture. Cytotechnology 31 (1999), 37–44.PubMedCrossRefGoogle Scholar
- 3.Zhang, G., Miura, Y., and Yagasaki, K.: Induction of apoptosis and cell cycle arrest in hepatoma cells by sera from tea-treated rats. Nutrition and Cancer 38 (2000), in press.Google Scholar
- 4.Yang, C.S., Chen, L., Lee, M.J., Balentine, D., Kuo, M.C. and Schantz, S.P.: Blood and urine levels of tea catechins after ingestion of different amounts of green tea by human volunteers. Cancer Epidemiol Biomarkers Prey 7 (1998), 351–354.Google Scholar
- 5.Akedo,.H., Shinkai, K., Mukai, M., Mori, Y., Tateishi, R., Tanaka, K., Yamamoto, R., and Morishita, T.: Interaction of rat ascites hepatoma cells with cultured mesothelial cell layers: a model for tumor invasion. Cancer Res 46 (1986), 2416–2422.Google Scholar
- 6.Shinkai, K., Mukai, M., and Akedo, H.: Superoxide radical potentiates invasive capacity of rat ascites hepatoma cells in vitro. Cancer lett 32 (1986), 7–13.CrossRefGoogle Scholar
- 7.Zhang, G., Miura, Y., and Yagasaki, K.: Suppression of adhesion and invasion of hepatoma cells in culture by tea compounds through antioxidative activity. Cancer Lett 152 (2000), 169–173.CrossRefGoogle Scholar