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Effect of β-Casein on Cytokine Production of Mouse Spleen Lymphocytes

  • Takeaki Okamoto
  • Hirofumi Tachibana
  • Koji Yamada
Conference paper
Part of the Animal Cell Technology: Basic & Applied Aspects book series (ANICELLTECH, volume 13)

Abstract

We showed β-Casein derived from bovine milk dose-dependently enhanced interferon (IFN)-γ and tumor necrosis factor (TNF)-α productions but not interleukin (IL)-4 production by mouse spleen lymphocytes. Furthermore, β-Casein increased the percentage of CD4+ T cell ratio but not CD8+ T cell ratio. That is, β-Casein significantly increased CD4+/CD8+ ratio. It is supposed from these results that β-Casein activates CD4+ T cells, especially Th1 type cells. And, the effects of β-Casein on the mRNA expressions of various cytokines in spleen lymphocytes were performed using RT-PCR method. RT-PCR analysis showed that spleen lymphocytes expressed the mRNA for IFN-γ, IL-2, IL-4, IL-6, IL-10 and IL-12p40 under Concanavalin A (ConA) stimulation. In addition, β-Casein stimulus increased the mRNA level of IFN-γ, IL-4 and IL-6 under ConA stimulation. And β-Casein stimulus increased the mRNA level of IL-12p40 under none stimulation. These results suggested that β-Casein activated not only IFN-γ production but also transcription activity of IFN-γ and spleen T cell response to the β-Casein is of the Th1 type. Moreover, the binding of the β-Casein to spleen lymphocytes was assessed by flow cytometory. β-Casein was strongly bound to some spleen lymphocytes, which were expected to be β-Casein sensitive population.

Keywords

Bovine Milk Spleen Lymphocyte Free Bile Acid HB4C5 Cell Caltag Laboratory 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media Dordrecht 2003

Authors and Affiliations

  • Takeaki Okamoto
    • 1
  • Hirofumi Tachibana
    • 1
  • Koji Yamada
    • 1
  1. 1.Laboratory of Food Chemistry, Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of AgricultureKyushu UniversityHigashi-ku, FukuokaJapan

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