Effect of β-Casein on Cytokine Production of Mouse Spleen Lymphocytes

  • Takeaki Okamoto
  • Hirofumi Tachibana
  • Koji Yamada
Conference paper
Part of the Animal Cell Technology: Basic & Applied Aspects book series (ANICELLTECH, volume 13)


We showed β-Casein derived from bovine milk dose-dependently enhanced interferon (IFN)-γ and tumor necrosis factor (TNF)-α productions but not interleukin (IL)-4 production by mouse spleen lymphocytes. Furthermore, β-Casein increased the percentage of CD4+ T cell ratio but not CD8+ T cell ratio. That is, β-Casein significantly increased CD4+/CD8+ ratio. It is supposed from these results that β-Casein activates CD4+ T cells, especially Th1 type cells. And, the effects of β-Casein on the mRNA expressions of various cytokines in spleen lymphocytes were performed using RT-PCR method. RT-PCR analysis showed that spleen lymphocytes expressed the mRNA for IFN-γ, IL-2, IL-4, IL-6, IL-10 and IL-12p40 under Concanavalin A (ConA) stimulation. In addition, β-Casein stimulus increased the mRNA level of IFN-γ, IL-4 and IL-6 under ConA stimulation. And β-Casein stimulus increased the mRNA level of IL-12p40 under none stimulation. These results suggested that β-Casein activated not only IFN-γ production but also transcription activity of IFN-γ and spleen T cell response to the β-Casein is of the Th1 type. Moreover, the binding of the β-Casein to spleen lymphocytes was assessed by flow cytometory. β-Casein was strongly bound to some spleen lymphocytes, which were expected to be β-Casein sensitive population.


Bovine Milk Spleen Lymphocyte Free Bile Acid HB4C5 Cell Caltag Laboratory 
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  1. 1,.
    Yamada K, Ikeda I, Nakajima H, Shirahata S and Murakami H (1991) Stimulation of proliferation and immunoglobulin production of human-human hybridoma by various types of caseins and their protease digests. Cytotechnology, 5, 279 – 285.PubMedCrossRefGoogle Scholar
  2. 2,.
    Takasugi M, Tamura Y, Tachibana H, Sugano M and Yamada K (2001) Development of assay system for immunoglobulin production regulatory factors using whole cell cultures of mouse splenocytes. Biosci. Biotechnol. Biochem., 65, 143 – 149.PubMedCrossRefGoogle Scholar
  3. 3,.
    Cher DJ and Mossman TR (1987) Two types of murine helper T cell clone II. Delayed-type hypersensitivity is mediated by Thl clone. J. Immunol., 138, 3688 – 3694.PubMedGoogle Scholar
  4. 4,.
    Firentino DF, Bond MW and MosmannTR (1989) Two types of mouse helper T cell. IV. Th2 clones secrete a factor that inhibits cytokine production by Thl clones. /. Exp. Med., 170, 2081 – 2095.CrossRefGoogle Scholar
  5. 5,.
    Satoru Nagata, Catriona McKenzie, Sylvia LP. Pender, Mona Bajaj-Elliott, Peter D. Fairclough, John A. Walker-Smith, Giovanni Monteleone, and Thomas T. MacDonald (2000) Human peyer’s patch T cells are sensitized to dietary antigen and display a Thl cell type 1 cytokine profile. J. Immunol165, 5315 – 5321.Google Scholar
  6. 6,.
    Lim BO, Yamada K, Yoshimura K, Watanabe T, Hung P, Taniguchi S and Sugano M (1995) Free bile acids inhibit IgE production by mouse spleen lymphocytes stimulated by lipopolysaccharide and interleukins. Biosci. Biotechnol. Biochem., 59, 624 – 627.PubMedCrossRefGoogle Scholar
  7. 7,.
    Mossman TR and Moore KW (1991) The role of IL-10 in crossregulation of Thl and Th2 responses. Immunol. Today, 12, A49 – A53.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2003

Authors and Affiliations

  • Takeaki Okamoto
    • 1
  • Hirofumi Tachibana
    • 1
  • Koji Yamada
    • 1
  1. 1.Laboratory of Food Chemistry, Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of AgricultureKyushu UniversityHigashi-ku, FukuokaJapan

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