Advertisement

Production of Pharmaceutical Proteins by Transgenic Avian

Conference paper
  • 332 Downloads
Part of the Animal Cell Technology: Basic & Applied Aspects book series (ANICELLTECH, volume 13)

Abstract

Transgenic avian has been expected to be an excellent transgenic bioreactor for the production of recombinant pharmaceutical proteins. However, successful transgenic bioreactors have been reported only in mammals. We have attempted to establish the generation method of transgenic avian using retroviral vectors for gene delivery. Laid quail embryos at the blastodermal stage were infected with a pantropic retroviral vector, and the embryos were hatched in vitro to generate G0 quails. The viral vector sequence was detected in the tissues of all G0 quails. The germ-line transmission efficiency of G0 quails mated with non-transgenic quails was more than 80%. Plural copies of the transgene were inserted into the genome of G1 transgenic progenies. The transgene expression was also detected in G1 and G2 transgenic quails.

Keywords

Vesicular Stomatitis Virus Viral Solution Transgenic Progeny Quail Embryo Blastodermal Stage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bosselmen, R.A., Hsu, R.V., Boggs, T., Hu, S.? Brusxewski, J., Ou, S., Kozar, L., Martin, F., Green, C., Jacobson, F., Nicolson, M., Schultz, J. A., Seman, K M., Rishell, W., and Stewart, R.G. (1989) Germline transmission of exogenous genes in the chicken. Science 243, 533–535.Google Scholar
  2. Burns, J.C., Friedmann, T., Driever, W., Burrascano, M., and Yee, J.K. (1993) Vesicular stomatitis virus G glycoprotein pseudotyped retroviral vectors: concentration to veiy high titer and efficient gene transfer into mammalian and nonmammalian cells. Proc. Natl Acad. Sci. USA 90, 8033–8037.PubMedCrossRefGoogle Scholar
  3. Cameron, E.E., Bachman, K.E., Myohanen, S., Herman, J.G., and Baylin, S.B. (1999) Synergy of demethylatoin and histone deacetylase inhibition in the re-expression of genes silenced in cancer. Nature Genet. 21, 103–107.PubMedCrossRefGoogle Scholar
  4. Dove. A. (2000) Milking the genome for profit. Nature Biotech. 18, 1045–1048.CrossRefGoogle Scholar
  5. Emi, N., Friedmann, T., and Yee, J.K. (1991) Pseudotype formation of murine leukemia virus with the G protein of vesicular stomatitis virus. J. Virol. 65, 1202–1207.PubMedGoogle Scholar
  6. Kamihira, M., Oguchi, S., Tachibana, A., Kitagawa, Y., and Iijima, S. (1998) Improved hatching for in vitro quail embryo culture using surrogate eggshell and artificial vessel. Develop. Growth Differ. 40, 449–55.CrossRefGoogle Scholar
  7. Love, J., Gribbin, C., Mather, C., and Sang, H. (1994) Transgenic birds by DNA microinjection. Bio/Technology 12. 60–63.PubMedCrossRefGoogle Scholar
  8. Rudolph, N.S. (1999) Biopharmaceutical production in transgenic livestock. Trends Biotechnol. 17, 367–374.PubMedCrossRefGoogle Scholar
  9. Salter, D.W., Smith, E.J., Hughes, S.H., Wright, S.E., and Crittenden, L.B. (1987) Transgenic chickens: insertion of retroviral genes into the chicken germ line. Virology 157, 236–240.PubMedCrossRefGoogle Scholar
  10. Sang, H. (1994) Transgenic chicken–methods and potential applications. Trends Biotechnol. 12, 415–420.PubMedCrossRefGoogle Scholar
  11. Ui, M., Takada, M., Arai, T., Matsumoto, K., Yamada, K., Nakahata, T., Nishiwaki, T., Furukawa, Y.,Tokino, T., Nakamura, Y., and Iba, H. (1999) Retrovirus vectors designed for efficient transduction of cytotoxic or cytostatic genes. Gene Ther. 6, 1670–1678.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2003

Authors and Affiliations

  1. 1.Department of Biotechnology, Graduate School of EngineeringNagoya UniversityChikusa-ku, NagoyaJapan

Personalised recommendations