Progress in Photosynthesis Research pp 557-560 | Cite as
Inhibition of Glycolate and D-Lactate Metabolism in a Mutant of Chlamydomonas Reinhardtii Deficient in Mitochondrial Respiration
Abstract
Glycolate dehydrogenase, an enzyme of the oxidative photosynthetic carbon cycle (C2 cycle), catalyzes the oxidation of glycolate to glyoxylate (1). This enzyme also reportedly catalyzes the oxidation of D-lactate (1). Unlike glycolate oxidase of higher plants, the dehydrogenase does not use molecular O2 as the direct electron acceptor; however, the natural acceptor is unknown. Both glycolate and D-lactate dehydrogenase activities have been cytochemically localized in the mitochondrial membrane (2) and glycolate-dependent reduction of cytochrome c by Chlamydomonas mitochondrial fractions has been reported (3). The present study describes the use of a mutant of Chlamydomonas reinhardtii deficient in cytochrome oxidase activity (4) to provide evidence that the metabolism of both glycolate and D-lactate in these algae is linked to mitochondrial electron transport.
Keywords
Wild Type Cell Mitochondrial Respiration Dark Respiration Rate Cytochrome Oxidase Activity Glycolate OxidasePreview
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References
- 1.Husic, D.W., Husic, H.D. and Tolbert, N.E. (1986) CRC Critical Reviews in Plant Sciences (in press).Google Scholar
- 2.Beezley, B.B., Gruber, P.J. and Frederick, S.E. (1976) Plant Physiol. 58, 315–319.CrossRefGoogle Scholar
- 3.Paul, J.S. and Vulcani, B.E. (1976) Planta 129, 59–61.CrossRefGoogle Scholar
- 4.Wiseman, A., Gillham, N.W. and Boynton, J.E. (1977) J. Cell Biol. 73, 56–77.CrossRefGoogle Scholar
- 5.Husic, D.W. and Tolbert, N.E. (1975) Plant Physiol. 78, 277–284.CrossRefGoogle Scholar
- 6.Sueoka, N. (1960) Proc. Natl. Acad. Sci. U.S.A. 46, 83–91.CrossRefGoogle Scholar
- 7.Nelson, E.B. and Tolbert, N.E. (1970) Arch. Biochem. Biophys. 141, 102–110.CrossRefGoogle Scholar
- 8.Wharton, D.C. and Tzagoloff, A. (1967) Meth. Enzymol. 10, 245–250.CrossRefGoogle Scholar
- 9.Calkins, V.P. (1943) Anal. Chem. 15, 762–763.Google Scholar
- 10.Tolbert, N.E., Husic, H.D., Husic, D.W., Moroney, J.V. and Wilson, B.J. (1985) in Inorganic Carbon Uptake by Aquatic Photosynthetic Organisms (Lucas, W.J. and Berry, J.A., eds.), pp. 221–227, Amer. Soc. of Plant Physiologists.Google Scholar
- 11.Tolbert, N.E., Harrison, M. and Selph, N. (1983) Plant Physiol. 72, 1075–1082.CrossRefGoogle Scholar
- 12.Moroney, J.V., Wilson, B.J. and Tolbert, N.E. (1986) Plant Physiol, (in press).Google Scholar