Obtaining Phases from Electron Microscopy for Solving Protein Structures

Tribute to Boris Vainshtein (1921–1996)
  • Sven Hovmöller
Chapter
Part of the NATO ASI Series book series (ASIC, volume 507)

Abstract

This paper is given in honour of Professor Boris Vainshtein, one of the founders of electron crystallography. He was an invited speaker at this NATO Advanced Study Institute on Electron Crystallography, but regrettably died half a year before it took place. Already in his classical book Structure Analysis by Electron Diffraction from 1964 [1] he wrote: “There is no doubt now that electron diffraction may be used for the complete analysis of crystals whose structure is unknown”.

Keywords

Cytochrome Oxidase Atomic Resolution Phase Problem Electron Diffraction Data NATO Advance Study Institute 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. 1.
    Vainshtein, B.K. (1964) Structure Analysis by Electron Diffraction. Pergamon Press, Oxford.Google Scholar
  2. 2.
    http://www.nobel.se/ Home page of the Nobel Foundation, Sweden
  3. 3.
    Hovmöller, S. (1992) Structure Determination of Inorganic Structures by Electron Microscopy and Crystallographic Image Processing. In: Electron Crystallography, Trans, of the American Crystallographic Association. Ed. D. Dorset. 28, 95–103.Google Scholar
  4. 4.
    Van Dyck, D. and de Beeck, M. (1992) Scanning Microscopy Supplement 6, 115.Google Scholar
  5. 5.
    BRITE-EURAM Project No. BE-3322 (1996) Ultramicroscopy 64 Entire volume.Google Scholar
  6. 6.
    Zou, X.D. (1995) Electron Crystallography of inorganic structures — Theory and practice Chem. Commun. 5. Stockholm University. Doctoral thesis.Google Scholar
  7. 7.
    Henderson, R. and Unwin, P.N.T. (1975) Three-dimensional model of purple membrane obtained by electron microscopy. Nature 257, 28–32.ADSCrossRefGoogle Scholar
  8. 8.
    Kühlbrandt, W., Wang, D.N., Fujiyoshi, Y. (1994) Atomic model of plant light-harvesting complex by electron crystallography Nature 367, 614–621.ADSCrossRefGoogle Scholar
  9. 9.
    Jap, B.K., Walian, P.J. and Gehring, K. (1991) Structure of porin Nature 350, 167-ADSCrossRefGoogle Scholar
  10. 10.
    Boekema, E.J., Van Heel, M.G. and Van Bruggen, E.F.J. (1984) Biochem. Biophys. Acta 787, 19–26.CrossRefGoogle Scholar
  11. 11.
    Brink, J., Hovmöller, S, Ragan, C.I., Cleeter, M.W.J., Boekema, E.J and van Bruggen, E.F.J. (1988) The Structure of NADH:Ubiquinone Oxidoreductase from Beef Heart Mitochondria. Crystals Containing an Octameric Arrangement of Iron-Sulphur Protein Fragments. Eur. J. Biochem. 166, 287–294.CrossRefGoogle Scholar
  12. 12.
    Leonard, K., Haiker, H. and Weiss, H. (1987) Three-dimensional structure of NADH:Ubiquinone reductase (Complex I) J.Mol.Biol. 194, 277–286.CrossRefGoogle Scholar
  13. 13.
    Henderson, R., Capaldi, R.A. and Leigh, J.S. (1977) Arrangement of cytochrome oxidase molecules in two-dimensional vesicle crystals. J.Mol.Biol. 112, 631–648.CrossRefGoogle Scholar
  14. 14.
    Hovmöller, S. (1990) Structure of cytochrome oxidase. An alternative interpretation. Manuscript submitted to J. Mol. Biol. but rejected.Google Scholar
  15. 15.
    Fuller, S.D., Capaldi, R.A. and Henderson, R. (1979) Structure of Cytochrome c Oxidase in Deoxycholate-derived Two-dimensional crystals. J. Mol. Biol. 134, 305–327.CrossRefGoogle Scholar
  16. 16.
    Tsukihara, T., Aoyama, H., Yamashita, E., Tomizaki, Yamaguchi, H., Shinzawa-Itoh, K., Nakashima, R., Yaono, R. and Yoshikawa, S. (1995) Structures of metal sites of oxidized bovine heart cytochrome c oxidase at 2.8 Å. Science 269, 1069–1074.ADSCrossRefGoogle Scholar
  17. 17.
    Leonard, K., Wingfield, P., Arad, T. and Weiss, H. (1981) Three-dimensional structure of Ubiquinolxytochrome c reductase from Neurospora mitochondria determined by electron microscopy of membrane crystals. J.Mol.Biol. 149, 259–274.CrossRefGoogle Scholar
  18. 18.
    Abrahams, J.P., Leslie, A.G.W., Lutter, R. and Walker, J. (1994) Structure at 2.8 Å resolution of F1- ATPase from bovine heart mitochondria. Nature 370, 621–628.ADSCrossRefGoogle Scholar
  19. 19.
    Van Heel, M. and Frank, J. (1981) Use of multivariate statistics in analysing the images of biological macromolecules. Ultramicroscopy 6, 187–194.Google Scholar
  20. 20.
    Evers, U., Franceschi, F., Boeddeker, N., Yonath, A. (1994) Crystallography of Haliphilic Ribosomes: the Isolation of an Internal Ribonucleoprotein Complex, Bioph. Chem., 50, 3–16.CrossRefGoogle Scholar
  21. 21.
    Vainshtein, B.K. and Pinsker, Z.G. (1950) Determination of the hydrogen positions in the crystal structure of a paraffin. Dokl. Akad. Nauk SSSR 72, 53–56.Google Scholar
  22. 22.
    Dorset, D. (1995) Structural Electron Crystallography, Plenum Press, New York.CrossRefGoogle Scholar
  23. 23.
    Weirich, T., Ramlau, R. Simon, A., Zou, X.D. and Hovmöller, S. (1996) A crystal structure determined to 0.02 Å accuracy by electron microscopy. Nature 382, 144–146.ADSCrossRefGoogle Scholar
  24. 24.
    Unwin, P.N.T. and Henderson, R. (1975) Molecular structure determination by electron microscopy of unstained crystalline specimens. J. Mol. Biol. 94, 425–440.CrossRefGoogle Scholar
  25. 25.
    Zou, X.D., Sundberg, M., Larine, M. and Hovmöller, S. (1996) Structure projection retrieval by image processing of HREM images taken under non-optimum defocus conditions. Ultramicroscopy 62, 103–121.CrossRefGoogle Scholar
  26. 26.
    Weiss, M.S., Abele, U., Weckesser, J. Weite, W. Schütz, E. and Schulz, G.E. (1991) Molecular architecture and electrostatic properties of a bacterial porin. Science 254, 1627–1630.ADSCrossRefGoogle Scholar
  27. 27.
    Avila-Sakar, A.J. and Chiu W. (1996) Visualization of β-sheets and side-chain clusters in two-dimensional periodic arrays of streptavidin on phospholipid monolayers by electron crystallography, Biophysical Journal 70, 57–68.ADSCrossRefGoogle Scholar
  28. 28.
    Akey, C.W., Spitsberg, V. and Edelstein, S.J. (1983) Electron microscopy of beef heart F1-ATPase crystals. J. Biol. Chem. 258, 3222–3229.Google Scholar

Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Sven Hovmöller
    • 1
  1. 1.Structural ChemistryStockholm UniversityStockholmSweden

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