Microbial Characterisation and Preparation of Inoculum for in vitro Mycorrhization of Strawberry in Autotrophic Culture

  • G. L. Mark
  • J. Murphy
  • Alan C. Cassells
Chapter
Part of the Developments in Plant Pathology book series (DIPP, volume 12)

Abstract

Strategies to control losses in vitro due to microbial contaminants (vitropaths [1]) have been the subject of extensive research (see Leifert and Woodward, this volume). Significant additional losses, however, may also occur at microplant establishment (Williamson et al., this volume). Polygenic disease resistance develops in seedlings as the seedling develops and it is recognised that seedlings are vulnerable to pathogens if growth is checked due to adverse environmental conditions [2]. Microplants, especially if vitrified (‘hyperhydrated’), may undergo a growth check at establishment [3]. Hardening of plants in vitro (‘in vitro weaning’) has been shown to improve plant establishment and subsequent growth [4] and should assist in reducing losses due to damping-off.

Keywords

Mycorrhizal Colonisation Heterotrophic Culture Streptomycin Sulphate Microbial Contaminant Autotrophic Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. 1.
    Herman, E.B. (1990) Acta Hortic. 280, 233–238.Google Scholar
  2. 2.
    Schumann, G.L. (1991) Plant Diseases: Their Biology and Social Impact, APS Press, St. Paul, USA.Google Scholar
  3. 3.
    Preece, J.E. and Sutter, E.G. (1991) in Micropropagation: Technology and Application (Debergh P.C. and Zimmerman R.H., eds) pp. 71–94, Kluwer, Dordrecht.CrossRefGoogle Scholar
  4. 4.
    Cassells, A.C. and Walsh, C. (1994) Plant Cell Tissue Org. Cult 37, 171–178.CrossRefGoogle Scholar
  5. 5.
    Cassells, A.C. and Tahmatsidou, V. (1996) Plant Cell Tissue Org. Cult. (in press).Google Scholar
  6. 6.
    Schönbeck, F., Grunewaldt-Stöcker, G. and Von Alten, H. (1994) in Epidemiology and Management of Root Diseases (Campbell C.L. and Benson D.M., eds) pp. 65–81, Springer-Verlag, Berlin, Germany.CrossRefGoogle Scholar
  7. 7.
    Wang, H., Parent, S., Gosselin, A. and Desjardins, Y. (1983) J. Am. Soc. Hortic. Sci. 118, 896–901.Google Scholar
  8. 8.
    Uosukainen, M. and Vestberg, M. (1994) Agric. Sci. Finl. 3, 269–279.Google Scholar
  9. 9.
    Mark, G.L. and Cassells, A.C. (1996) Plant Soil 185, 233–239.CrossRefGoogle Scholar
  10. 10.
    Schenck, N.C., Graham, S.O. and Green, N.E. (1975) Mycologia 67, 1189–1192.PubMedCrossRefGoogle Scholar
  11. 11.
    Tommerup, I.C. (1985) Trans. Br. Mycol. Soc. 85, 267–278.CrossRefGoogle Scholar
  12. 12.
    Cassells, A.C. and Mark, G.L. (1996) Agronomie 16, 563–571.CrossRefGoogle Scholar
  13. 13.
    Schaad, N.W. (1980) Laboratory Guide for Identification of Plant Pathogenic Bacteria, APS Press, St. Paul, MN, USA.Google Scholar
  14. 14.
    Murashige, T. and Skoog, F. (1962) Physiol. Plant. 15, 473–497.CrossRefGoogle Scholar
  15. 15.
    Philips, J.M. and Hayman, D.S. (1970) Trans. Br. Mycol. Soc. 55, 158–160.CrossRefGoogle Scholar
  16. 16.
    Giovanetti, M. and Mosse, B. (1980) New Phytol. 84, 489–500.CrossRefGoogle Scholar
  17. 17.
    Lovato, P.N., Gianinazzi-Pearson, V., Trouvelot, A. and Gianinazzi, S. (1996) Adv. Hortic. Sci., 10, 46–52.Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 1997

Authors and Affiliations

  • G. L. Mark
    • 1
  • J. Murphy
    • 1
  • Alan C. Cassells
    • 1
  1. 1.Department of Plant ScienceUniversity CollegeCorkIreland

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