Low temperature storage of plant tissue cultures

  • Brian W. W. Grout
Chapter

Abstract

Micropropagation is firmly established as a global technology for plant production systems and is widely used in teaching and research as well as, on the larger scale, for commercial production. It is extremely labour-intensive for a modern technology, as the interval between subcultures may range, typically, from 14 to 35 days. This creates a high, continual demand for skilled labour, media, culture vessels and space in the specialised environmental conditions required for optimal growth. In any micropropagation facility, whatever its size, there will be a high proportion of material being held for some future use, and which is not part of a current multiplication or experimental programme. Consequently, there will be significant logistic and resource benefits in employing a storage system for this material, to extend significantly the intervals between subculture and other handling. Resource savings will also be made if the storage system can operate at lower temperatures and light levels than those required for optimal growth. Programmed use of such a storage system would also allow greater control of production schedules for micropropagated material, with efficient stockpiling of multiplying cultures or plantlets.

Keywords

Somatic Embryo Cold Acclimation Plant Tissue Culture Plant Genetic Resource Chilling Injury 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer Science+Business Media Dordrecht 1995

Authors and Affiliations

  • Brian W. W. Grout
    • 1
  1. 1.Crisp Innovar Ltd.Glebe HouseReepham, NorfolkUK

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