Advertisement

UDP-Galactose: Diacylglycerol Galactosyltransferase in Cucumber Seedlings: Purification of the Enzyme and the Activation by Phosphatidic Acid

  • Hiroyuki Ohta
  • Mie Shimojima
  • Takeshi Arai
  • Tatsuru Masuda
  • Yuzo Shioi
  • Ken-ichiro Takamiya
Chapter

Abstract

UDP-galactose:diacylglycerol galactosyltransferase (UDGT) catalyzes the final step of the synthesis of monogalactosyl diacylglycerol (MGDG), a major glycohpid in plastid membrane. We found that the enzyme activity was strongly activated coincided with accumulation of galactolipids during germination of cucumber seedlings, suggesting that activation step of the UDGT was important in the development of chloroplast membrane [1]. However, mechanism of regulation of the UDGT activity during chloroplast formation remains unclear.

Keywords

Phosphatidic Acid Phosphatidic Acid Lyso Phosphatidic Acid Cucumber Seedling Chloroplast Membrane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    Ohta H, Shimojima M, Ookata K, Masuda T, Shioi Y, Takamiya K. A galactosyl-transferase is activated in light-mediated accumulation of galactolipids during development in cucumber seedlings. 1994;submitted.Google Scholar
  2. [2]
    Teucher, T and Heinz, E. Punfication of UDP-galactose:diacylglycerol galactosyl-transferase from chloroplast envelopes of spinach (Spinacia oleracea L.) Planta 1991;184:319–326.CrossRefGoogle Scholar
  3. [3]
    Maréchalé, Block MA, Joyard J, Douce R. Purification of UDP:galactose 1.2-diacylglycerol galactosyltransferase from spinach chloroplast envelope mem-branes.C. R. Acad. Sci. Paris, t. 313, Série III, 1991;521–528.Google Scholar
  4. [4]
    Coves J, Joyard J, Douce R. Lipid requirement and kinetic studies of solubilized UDP-galactose:diacylglycerol galactosyltransferase activity from spinach chloroplast envelope membranes. Proc. Natl Acad .Sci. USA 1988;85:4966–4970.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 1995

Authors and Affiliations

  • Hiroyuki Ohta
    • 1
  • Mie Shimojima
    • 1
  • Takeshi Arai
    • 1
  • Tatsuru Masuda
    • 1
  • Yuzo Shioi
    • 1
  • Ken-ichiro Takamiya
    • 1
  1. 1.Faculty of Bioscience & BiotechnologyTokyo Institute of TechnologyNagatsuta, Yokohama 227Japan

Personalised recommendations