Abstract
Oil body boundary proteins (so called Oleosins) have been extensively studied and many investigations have dealt with their molecular characterisation [1,2]. These proteins are highly hydrophobic. Little work, therefore, has been carried out on their purification for chemical and physical studies. Previous isolation procedures have utilised the differential density of the lipid in the oil-body to separate an oil-body fraction from the rest of the cell. This leads to contamination by many other cellular hydrophobic proteins. Here we present a protocol for the rapid purification of sunflower oil-body proteins, and assess some of their physical properties.
Keywords
Lipid Body Crude Homogenate Urea Buffer Rapid Purification Differential Density
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References
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© Springer Science+Business Media Dordrecht 1995