Expression of Acyl-[ACP] Thioesterase in Cuphea Lanceolata and in Transgenic Rapeseed

  • Norbert Martini
  • Jeff Schell
  • Reinhard Töpfer


A comparison of deduced amino acid sequences from 10 plant acyl-[Acyl Carrier Protein] thioesterases í TE) and their relationship to six Cuphea TEs is discussed. Expression studies of one TE from Cuphea lanceolata (C/TE13) in roots, flowers, leaves, maturing embryos and seeds showed a preferred expression in flowers. Heterologous expression in transgenic Brassica napus revealed 3 % capric acid in immature seeds of the T2 progeny.


Capric Acid Acyl Carrier Protein Active Site Cysteine Transgenic Rapeseed Storage Triacylglycerols 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Harwood JL. Fatty acid metabolism. Ann Rev Plant Physiol Plant Mol Biol 1988; 39:101–138.CrossRefGoogle Scholar
  2. 2.
    Voelker TA. Worrell AC. Anderson L et al. Fatty acid biosynthesis redirected to medium chains in transgenic oilseed plants. Science 1992; 257: 72–74.PubMedCrossRefGoogle Scholar
  3. 3.
    Dormann P, Spener F., Ohlrogge JB. Characterization of two acyl-acyl carrier protein thioesterases from developing Cuphea seeds specific for medium-chain- and oleoyl-acyl carrier protein. Planta 1993; 189: 425–432.CrossRefGoogle Scholar
  4. 4.
    Schuch R, Brummel M., Spener F. Medium-chain acyl-ACP thioesterase is not the exclusive enzyme responsible for early chain-length termination in medium-chain fatty acid synthesis. Grasas y Aceites 1993; 44:126–128.Google Scholar
  5. 5.
    Töpfer R., Martini N. Molecular cloning of cDNAs or genes encoding proteins involved in de novo fatty acid biosynthesis in plants. J Plant Physiol 1994; 143:416–425.CrossRefGoogle Scholar
  6. 6.
    Knutzon DS. Bleibaum JL, Nelsen J, Kridl JC, Thompson GA. Isolation and characterization of two safflo-wer oleoyl-acyl carrier protein thioesterase cDNA clones. Plant Physiol 1992; 100:1751–1758.PubMedCrossRefGoogle Scholar
  7. 7.
    Smeekens S, Weisbeek P, Robinson C. Protein transport into and within chloroplasts. Trends Biochem Sci 1990; 15:73–76.PubMedCrossRefGoogle Scholar
  8. 8.
    Ohlrogge JB, Kuhn DN, Stumpf PK. Subcellular localization of acyl carrier protein in leaf protoplasts of Spinacia oleracea. Proc Nat Acad Sci USA 1979; 76:1194–1198.PubMedCrossRefGoogle Scholar
  9. 9.
    Voelker TA, Davies HM. Plant medium-chain thioesterases. International Patent Application 1992; WO 92/20236.Google Scholar
  10. 10.
    de Silva J, Robinson SJ, Safford R. The isolation and functional characterization of a B. napus acul carrier protein 5 ‘ flanking region involved in the regulation of seed storage lipid synthesis. Plant Mol Biol 1992; 18: 1163–1172.PubMedCrossRefGoogle Scholar
  11. 11.
    Voetz M. Töpfer R. Köln, 1992; unpublished data.Google Scholar
  12. 12.
    Töpfer R, Maas C, Höricke-Grandpierre C, Schell J, Steinbiss HH. Expression vectors for high-level gene expression in dicotyledonous and monocotyledonous plants. In: Wu R, editor. Recombinant DNA. Methods in Enzymology. San Diego: Academic Press, Inc. 1993; 217:66–78.Google Scholar
  13. 13.
    Loader NM, Woolner EM, Hellyer A, Slabas AR, Safford R. Isolation and characterization of two Brassica napus embryo acyl-ACP thioesterase cDNA clones. Plant Mol Biol 1993; 23: 769–778.PubMedCrossRefGoogle Scholar
  14. 14.
    Dörmann P, Kridl JC, Ohlrogge JB. Cloning and expression in Escherichia coli of a cDNA coding for the oleoyl-acyl carrier protein thioesterase from coriander seed endosperm (Plant Gene Register). Genbank accession number: L20978.Google Scholar
  15. 15.
    Grellet F, Cooke R, Raynal M, Laudie M, Delseny M. Arabidopsis thaliana systematic cDNA sequencing reveals a gene with homology with Umbellularia californica C12:0-ACP thioesterase. Plant Physiol Biochem. 1993; 31: 599–602.Google Scholar
  16. 16.
    Devereux J, Haeberli P, Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucl Acids Res 1984; 12:387–395.PubMedCrossRefGoogle Scholar
  17. 17.
    Leonard J, Slabaugh M, Knapp S J. Corvallis, 1993; unpublished data.Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 1995

Authors and Affiliations

  • Norbert Martini
    • 1
  • Jeff Schell
    • 1
  • Reinhard Töpfer
    • 1
  1. 1.Max-Planck-Institut für ZüchtungsforschungKölnGermany

Personalised recommendations