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Post-transcriptional Regulation of Storage Protein Synthesis during Early Embryogenesis

  • J. D. Bewley
  • S. K. Pramanik
Part of the Current Plant Science and Biotechnology in Agriculture book series (PSBA, volume 30)

Abstract

Messenger RNA synthesis during somatic embryogenesis of alfalfa is controlled at the post-transcriptional level during the globular to torpedo stages of development, and transcriptionally during the cotyledonary stages. Storage protein messages are sequestered in messenger ribonucleoprotein (mRNP) complexes during the early stages of embryogenesis, in association with specific binding proteins. Reconstitution of the messenger-protein complex can be achieved in vitro using medicagin (11S) mRNA; cell-free extracts from pre-cotyledonary embryos contain different binding proteins from those at the cotyledonary stages. Those associated with mRNA at the early stages may prevent translation, whereas those at the late stages may enhance large polysome formation. The mRNA-bound proteins from pre-cotyledonary-stage embryos exhibit 50-fold more protein kinase activity than those from cotyledonary embryos, and several proteins in the mRNP fraction can be phosphorylated. While the physiological function of the phosphorylated proteins is unknown, it is possible that selective phosphorylation and dephosphorylation of proteins determines recruitment of individual mRNAs into polysomes, thus regulating translation during embryogenesis.

Keywords

Somatic Embryo Somatic Embryogenesis Storage Protein Zygotic Embryo Cotyledonary Stage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bewley, J. D., Pramanik, S. K. and Krochko, J. E 1993. In: Basic and Applied Aspects of Seed Biology. Proceedings of the Fourth International Workshop on Seeds, pp. 29–38 (eds. D. Côme and F. Corbineau). Paris: ASFIS.Google Scholar
  2. Bewley, J. D. and Marcus, A. 1990. Progress in Nucleic Acids Research and Molecular Biology 38: 165–193.CrossRefGoogle Scholar
  3. Blenis, J., Kuo, C. J. and Erikson, R. L. 1987. Journal of Biological Chemistry 262: 14373–14376.PubMedGoogle Scholar
  4. Coulter, K. M. and Bewley, J. D. 1990. Journal of Experimental Botany 41: 1541–1547.CrossRefGoogle Scholar
  5. Dreyfuss, G. 1986. Annual Reviews of Cell Biology 2: 459–498.CrossRefGoogle Scholar
  6. Hershey, J. W. B. 1990. Annual Reviews of Biochemistry 60: 717–755.CrossRefGoogle Scholar
  7. Jeno, P., Balou, L. M., Novak-Hofer, I. and Thomas, G. 1988. Proceedings of the National Academy of Science U.S.A. 85: 406–410.CrossRefGoogle Scholar
  8. Krochko, J. E. and Bewley, J. D. 1990. Journal of Experimental Botany 41: 505–514.CrossRefGoogle Scholar
  9. Krochko, J. E., Pramanik, S. K. and Bewley, J. D. 1992. Plant Physiology 99: 46–53.PubMedCrossRefGoogle Scholar
  10. Morley, S. J. and Traugh, J. A. 1989. Journal of Biological Chemistry 264: 2401–2404.PubMedGoogle Scholar
  11. Pramanik, S. K., Krochko, J. E. and Bewley, I D. 1992. Plant Physiology 99: 1590–1596.PubMedCrossRefGoogle Scholar
  12. Prebrazhensky, A. A. and Spirin, A. S. 1978. Progress in Nucleic Acids Research and Molecular Biology 21: 1–38.CrossRefGoogle Scholar
  13. Price, D. I, Nemeoff, R. A. and Aurach, J. 1989. Journal of Biological Chemistry 264: 13825–13833.PubMedGoogle Scholar
  14. Proud, C G. 1992. Current Topics in Cellular Regulation 32: 243–269.PubMedGoogle Scholar
  15. Rosenthal, E. T., Hunt, T. and Rudeman, J. V. 1980. Cell 20: 487–494.PubMedCrossRefGoogle Scholar
  16. Smith, K. C. 1976. Photochemistry and Photobiology of Nucleic Acids 2: 187–212.CrossRefGoogle Scholar
  17. Xu, N., Coulter, K. M., Krochko, J. E. and Bewley, I D. 1991. Seed Science Research 1: 119–125.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 1997

Authors and Affiliations

  • J. D. Bewley
    • 1
  • S. K. Pramanik
    • 1
  1. 1.Department of BotanyUniversity of GuelphGuelphCanada

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