Study for the Production of a Recombinant Monoclonal Antibody in Stirred Tank

Abstract

Stirred tank is one of the main techniques for scaling-up cultures for production of recombinant proteins for therapeutical use. A study of culture conditions for the production of a humanized monoclonal antibody against EFG receptor was performed. A basal medium selection was done. The influence of stirring in cell growth was tested. The result showed that there have not influence until 150 rpm in 250 ml spinners flask. An extrapolation to higher volume vessel let velocities of 400 rpm without cell damage was possible.

Paralel determination of oxygen transfer for different levels of gas flow and stirring was done.

A series of suspension batch cultures in spinners flasks and in 4 1 fermenter were made with the prestablished conditions, the cell growth and productivity were improved.