Viral Contaminants Found in Mouse Cell Lines Used in the Production of Biological Products

  • Stephen J. Froud
  • John Birch
  • Carol McLean
  • Alasdair J. Shepherd
  • Kenneth T. Smith

Abstract

Murine hybridoma and plasmacytoma cell lines are used in the production of biologics for human use such as therapeutic monoclonal antibodies and recombinant proteins. Most of these cell lines produce retro virus-like particles and in many cases infectious C type murine leukaemia virus (MLV) which can be either ecotropic (infectious for cell lines of murine origin or a closely related species) or xenotropic (infectious for species other than murine; X-MLV).

Keywords

Reverse Transcriptase Activity Mouse Cell Line Viable Cell Concentration Reverse Transcriptase Assay MOPC21 Tumour 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media Dordrecht 1997

Authors and Affiliations

  • Stephen J. Froud
    • 1
  • John Birch
    • 1
  • Carol McLean
    • 2
  • Alasdair J. Shepherd
    • 2
  • Kenneth T. Smith
    • 2
  1. 1.Lonza Biologics (previously Celltech Biologics)Slough, BerkshireUK
  2. 2.Q-One Biotech Ltd.GlasgowUK

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