Micro Total Analysis Systems ’98 pp 343-346 | Cite as
Microfabricated Dual-Microdialysis and Capillary Isoelectric Focusing Devices for Cleanup and Separations / Mass Spectrometric Analysis of Biomolecules
Conference paper
Abstract
A laser microfabricated device was constructed for rapid on-line microdialysis cleanup of biological samples for analysis by electrospray ionization mass spectrometry (ESI-MS). Efficient desalting was achieved for protein samples using ESI with an ion trap mass spectrometer. Signal-to noise ratios were greatly enhanced compared to direct infusion of the original nondialyzed samples. Microfabricated capillary isoelectric focusing (CIEF) devices were also constructed and successful separations of various protein samples with the CIEF chip were demonstrated.
Keywords
microdialysis electrospray ionization mass spectrometry capillary isoelectric focusing proteinPreview
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References
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Copyright information
© Springer Science+Business Media Dordrecht 1998