Animal Cell Technology: Basic & Applied Aspects pp 349-354 | Cite as
Development of High Output Expression Vectors for Antibody Production in Mammalian Cells
Chapter
Abstract
The design of efficient expression vectors is a major component in the development of a high level production system for recombinant antibodies. The BKV enhancer/Adenovims major-late promoter (BKV/AdMLP) and human cytomegalovims immediate-early enhancer/promoter (CMV) were evaluated. The Adenovirus tripartite leader containing a chimeric intron (AdTpl-SS), immunoglobulin leader intron and J/C chimeric intron were aided and/or shifted. Cell lines COS-1, CV-1, 293S, CHO and NSO were used as hosts for transient assays. The secreted products were quantified by ELISA.
Keywords
Splice Site Expression Cassette Recombinant Antibody Transient Assay Kappa Light Chain
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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© Springer Science+Business Media Dordrecht 1998