Pharmacodynamics and localization of 3-devinyl-3-formylchlorin p6 in living cancer cells as studied with confocal spectral imaging (CSI) technique

Abstract

The CSI technique comprises the measurements of a 2D set of intracellular fluorescence spectra of an antitumor agent with a 3D confocal resolution and subsequent decomposition of these spectra into models describing different states and interactions of the drug [1, 2]. The models have to be taken from in vitro experiments. The results of the decomposition present themselves as maps of intracellular distribution of complexes and states of the agent. Each point of the cell can be characterized in terms of drug concentration after calibration procedure. This allows one to determine regions/compartments of predominant drug accumulation and to predict probable targets of its antitumor action.