Photophysics of P-pyridoxyl amino acids. Time-resolved fluorescence study and molecular modelling

  • V. Stepuro
  • S. Maskevich
Chapter

Abstract

It is known that pyridoxal-5′-phosphate (PLP), the biologically active coenzymic form of the vitamin B6 compounds, can modify proteins and influence their functional states and properties [1]. Carbonyl groups of PLP interact with amino groups to produce Schiff bases. When aldimine bond of the Schiff base is reduced stable adduct (secondary amine) is formed. In this work the properties of PLP as a possible fluorescent label for proteins were studied. Time-resolved fluorescence studies and PM3 semi-empirical calculations were carried out with model compounds (P-pyridoxyl amino acids).

Keywords

Schiff Base Aromatic Amino Acid Fluorescence Decay Aliphatic Amino Acid Fluorescence Kinetic 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. 1.
    Benesch, R.E., Benesch, R., Renthal, R.D., and Maeda N., Biochemistry, 11 (1972) 3576.CrossRefGoogle Scholar
  2. 2.
    Gachko, G.A., Zybelt, V.K., Kivach, L.N., Maskevich, S.A., Maskevich, A.A., Zhumal Prikl. Spect. (Russ), 47 (1987) 335.Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 1999

Authors and Affiliations

  • V. Stepuro
    • 1
  • S. Maskevich
    • 1
  1. 1.Yanka Kupala State UniversityGrodnoBelarus

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