The Improvement of Heavy Metal Tolerance in Cyanobacteria by Gene Engineering

  • Ding-Ji Shi
  • Jun Sun
  • Ping Jin
  • Xiang-Xue Guo
  • Li Ren
  • Xu-Dong Xu
  • Binggen Ru
Chapter

Abstract

Heavy metal pollution has been become the most serious in irrigated area of China with waste water, occupying about 64% the area[1], These heavy metals, such as cadmium(Cd), mercury(Hg) and lead(Pb), can enter into the food chain and do great harm to human health. Cyanobacteria have been found their capability of riching heavy metals in marine and they possess unique adaptation to various ecosystems. More and more people consider that cyanobacteria are biotechnically potential environmental bioremediator[2,3].

Key words

heavy metals tolerance transgenic cyanobacteria gene expression metalloproteins(metallothioneins) 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1].
    Xu JL & Yang JR (eds.) 1995. Heavy Metals in Land Ecosystems. Chinese Press of Environment Sciences. pp 1–449.Google Scholar
  2. [2].
    Sun J, Jin P, Xu XD, Shi DJ, Li LY. & Ru BG. 1994 Progress in Biotechnology (chinese), 14(6):39–42.Google Scholar
  3. [3].
    Erbe JL, Taylor KB & Hall LM. 1996. J. Ind: Microbiol. 17:41–46.Google Scholar
  4. [4].
    Vallee BL, 1991. Methods Enzymol. 205:3–7.CrossRefPubMedGoogle Scholar
  5. [5].
    Olafson RW, McCullin WD & Kay CM. 1988. Biochem.J. 251, 691–699.CrossRefPubMedPubMedCentralGoogle Scholar
  6. [6].
    Guo XX, Chen ZJ, Dan CT, Shi DJ & Ru BG. 1997. Chinese J.Biochem 13(6):699–703.Google Scholar
  7. [7].
    Ren L, Shi DJ, Dai JX & Ru BG. 1998. FEMS Microbiol. Letts. 158:127–132CrossRefGoogle Scholar
  8. [8].
    Guo XX, Zhao H, Shi DJ, Xu J, Xu XX & Ru BG 1998. Acta Bot. Sin. 40(4)320–324.Google Scholar
  9. [9].
    Castenholz RW 1988. Methods Enzymol. 167:68–93.CrossRefGoogle Scholar
  10. [10].
    Elhai J. & Wolk CP. 1988. Methods Enzymol.167:747–754.CrossRefPubMedGoogle Scholar
  11. [11].
    Goyal D. 1992. J.Microbiol. Methods 15:7–15.CrossRefGoogle Scholar
  12. [12].
    Sambrook K, Fritsch EF & Maniatis T 1989. Moloecular Cloing:A Laboratory Manual. znd ed. Cold Spring Harbor Laboratory Press. New York.Google Scholar
  13. [13].
    Briggs RW & Armitage IM 1982. J. Bio. chem. 257: 1259–1262.Google Scholar
  14. [14].
    Shi DJ 1987. Energy Metabolism and Structure of Immobilized Cyanobacterium Anabaena azollae. Ph.D. thesis, University of London. pp 1–381Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 1998

Authors and Affiliations

  • Ding-Ji Shi
    • 1
  • Jun Sun
    • 2
  • Ping Jin
    • 1
  • Xiang-Xue Guo
    • 2
  • Li Ren
    • 3
  • Xu-Dong Xu
    • 4
  • Binggen Ru
    • 2
  1. 1.Institute of BotanyChinese Academy of SciencesBejingChina
  2. 2.Life Sicience CollegePeking UniversityBeijingChina
  3. 3.Marine University of QingdaoQingdaoChina
  4. 4.MSU-DOE Plant Research Lab. Michigan State UniversityMichiganUSA

Personalised recommendations