Epithelia pp 333-350 | Cite as

Cell Culture Systems for the Study of Human Skin and Skin Glands

  • C. M. Lee
Chapter

Abstract

Cell culture — the maintenance of a population in vitro through at least one cycle of division — has a long, somewhat tortuous, history. Although successful attempts to grow animal cells in vitro were made as early as the beginning of the century (e.g. References 1 and 2), there were major problems with microbial contamination and irreproducibility of experiments, the latter of which arose from the use of undefined natural fluids as culture media. Despite these problems, the new technique was extended to a variety of cell types via an imaginative range of biological supplements, including lymphatic, amniotic and spinal fluids, plasma, serum and various tissue and embryo extracts. The minimization of contamination and irreproducibility, however, continued to require a vigorous approach, and technical progress over the next 35 years was limited to minor refinements of very demanding experimental protocols.

Keywords

Cystic Fibrosis Sweat Gland Sebaceous Gland Bullous Pemphigoid Human Epidermal Keratinocytes 
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