Summary
F(ab’)2 fragments of an anti-CEA monoclonal antibody were radiolabelled with 123-I and used to study eleven patients with various types of gastrointestinal carcinomas. The antibody scans detected primary or metastatic disease in 4 out of 7 patients with known disease and they suggested the existence of disease in 3 cases, in sites not detected by conventional techniques. In 1 patient, liver metastases were detected with the antibody scan 3 months earlier than with CT scan examination. Absence of disease was confirmed in 3 patients in complete remission. These results indicate that external scintigraphy using 123-I labelled F(ab’)2 fragments of anti-CEA monoclonal antibodies may be a useful tool in monitoring patients with gastrointestinal tumours in terms of confirming the results of conventional methods or revealing disease sites undetected by conventional radiology.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Begent R.H.K. et al (1980) Radioimmunolocalisationof tumours by external scintigraphy after administration of 131-I antibody to human chronic gonadotropin: preliminary communication. J. Royl. Soc. Med. 73: 624–630
Berche C. et al(1982) Tomoscintigraphyfor detecting gastrointestinaland medullary thyroid cancers: first clinical results using radiolabelled monoclonal antibodies against carcino embryonic antigen. Br. Med. J. 285: 1447–1451
Chatal J.F. et al (1984) Immunoscintigraphyof colon carcinoma. J. Nucl. Med. 25: 307–314
Dykes D.W. et al (1980) Localisation of tumour deposits by external scanning after injection of radiolabelled anti-carcinoembryonicantigen Br.Med.J. 280: 220–222
Delaloye B. et al (1985) First approach to therapy of liver metastases in colorectal carcinoma by intrahepatically infused 131-I labelled monoclonal anti-CEA antibodies. Eur. J. Nucl. Med. 11:A37
Delaloye B et al (1986) Detection of colorectal carcinoma by emission computerised tomography after injection of 123-I labelled Fab and F(ab’)2 fragments from monoclonal anti-carcinoembryonicantigen antibodies. J.Clin.Inv. 77: 301–311
Endo T. et al (1985) Immunohistologic distribution of CEA defined by human anti-CEA antibody in the serum of a colonic cancer patient. Cancer 56: 2205–2211
Epenetos A.A, et al (1984) Antibody guided irradiationof malignant lesions. Lancet 1: 1441–1443
Fritzberg AR. et al (1988) Specific and stable labelling of antibodies with technetium-99m with a diamide dithiolate chelating agent. Proc. Natl. Acad. Sci. USA 85: 4025–4029
Goldenberg D. et al (1978) Use of radiolabelled antibodies to carcinoembryonic antigen for the detection and localisation of diverse cancers by external photoscanning. New Engl. J. Med. 298: 1384–1388
Kalofonos H.P. et al (1988) Kinetics, quantitativeanalysis and radioimmunolocalisationusing indium-111HMFG1 monoclonal antibody in patients with breast cancer. Br. J. Cancer 59: 934–942
Kohler G and Milstein C (1975) Continuous culture of fused cells secreting antibody of predefined specificity. Nature 256: 465–467
Mach J.P et al (1980) Tumour localisation of radiolabelled antibodies against carcinoembryonic antigen in patients with carcinoma. New Engl. J. Med. 305: 5–10
Mach J.P. et al (1981) Use of 131-I radiolabelled monoclonal anti-CEA antibodies for the detectionof human carcinomas by external photoscanningand tomoscintigraphy. Immunology Today 2: 239–249
Pectasides D. et al (1986) Antibody guided irradiation of malignant pleural and pericardial effusions. Br. J. Cancer 53: 727–732
Reay P. (1982) Use of N-bromosuccinimide for the iodination of proteins for radioimmunoassay. Ann. Clin. Biochem. 19: 129–133
Riva P et al (1988) Antibody-guided diagnosis an Italian experience on CEA-expressing tumours In. J. Cancer Supplement 2: 114–120
Siccardi A.G. et al (1989) Immunoscintigraphy of adenocarcinomas by means of radiolabelled F(ab’)2 fragments of an anti-carcinoembryonicantigen monoclonal antibody: a multicentre study. Cancer Res. 49: 3095–3103.
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Bamias, A. et al. (1991). Use of 123-I Radiolabelled F(AB’)2 Fragments of Anti-CEA Monoclonal Antibody for the Detection of Primary and Metastatic Gastrointestinal Carcinomas. In: Baum, R.P., Cox, P.H., Hör, G., Buraggi, G.L. (eds) Clinical Use of Antibodies. Developments in Nuclear Medicine, vol 19. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3516-0_5
Download citation
DOI: https://doi.org/10.1007/978-94-011-3516-0_5
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-5555-0
Online ISBN: 978-94-011-3516-0
eBook Packages: Springer Book Archive