Nitrogen Fixation pp 513-517 | Cite as
Structural and Genetic Studies on Four Ferredoxins from the Photosynthetic Bacterium Rhodobacter Capsulatus; Probable involvement in Nitrogen Fixation
Chapter
Abstract
Four ferredoxins have been identified and chemically characterized in Rhodobacter capsulatus. The possible function of these ferredoxins has been investigated by means of in vitro biological assays and genetic studies. The primary structure of each of the four ferredoxins has been determined. The nature of their iron-sulfur clusters was established using UV-visible and EPR spectroscopy. FdI and FdIII contain two [4Fe-4S] clusters, FdII contains both one [3Fe-4S] and one [4Fe-4S] cluster, whereas FdIV has a [2Fe-2S] cluster similar to plant-type ferredoxins. The structural gene of FdI, fdxN, was cloned, sequenced and shown to be part of a group of nif genes. The gene encoding FdIV, fdxC, was localized just upstream of fdxN. The gene coding for FdIII, fdxB, has been found to be part of an operon containing three nif genes (nifE, nifN, nifQ) known to be involved in the FeMo-co synthesis. The transcription of fdxB, fdxC and fdxN is inhibited in cells grown with excess NH4 +. The gene encoding FdII was cloned and sequenced. This gene, called fdxA, is regulated independently of nif genes.
Keywords
Nitrogen Fixation Photosynthetic Bacterium Rhodospirillum Rubrum Rhodobacter Capsulatus Physiological Electron Donor
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© Springer Science+Business Media Dordrecht 1991