Virus Purification

  • David G. A. Walkey
Chapter

Abstract

The information that can be obtained about any plant virus is limited, if the investigation is confined to an ‘in vivo’ study. To obtain information on many biochemical and physical properties, and to produce antisera for serological studies (see Section 6.6), it is necessary for the virus particles to be separated from the host and concentrated ‘in vitro’ This process is referred to as purification, and for new viruses in particular, it is essential for characterization and identification. The purified virus should be physically and chemically undamaged by the purification procedure and free from contaminating host-material.

Keywords

Tobacco Mosaic Virus Potato Virus Cucumber Mosaic Virus Plant Virus Sodium Sulphite 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Further Selected Reading

  1. Brakke, M. K. (1967). Density gradient centrifugation, pp. 93–118; Miscellaneous problems in virus purification, pp. 119–36. In Methods in virology (ed. Maramorosch, K. and Koprowski, H.), Academic Press: New York.Google Scholar
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  3. Matthews, R. E. F. (1981). Plant virology. Academic Press: New York.Google Scholar

Copyright information

© David G. A. Walkey 1991

Authors and Affiliations

  • David G. A. Walkey
    • 1
    • 2
  1. 1.Institute of Horticultural ResearchWellesbourneUK
  2. 2.Plant Science DepartmentUniversity CollegeCardiffUK

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