Abstract
Several T-DNA genes involved in opine biosynthesis are efficiently transcribed in plant tumor tissue (Karcher et al., 1984). The presence of eucaryotic transcription signals flanking these Agrobacterium tumefaciens genes, facilitates their recognition by the plant transcriptional machinery. Several of the T-DNA promoters are therefore frequently used for expression of foreign genes in transgenic plants. Two of these promoters are the closely linked mannopine synthase (mas) promoters derived from the TR-DNA of an octopinetype Ti plasmid. Only 0.36 kb of DNA separates the start points of transcription for this bidirectional promoter region, which can be used to control the expression of two gene products simultaneously. Of vital importance for the construction of chimeric genes to be expressed in a desired plant tissue or at a certain stage of development, is the characterization of organ and cell-specific promoters for driving genes of interest.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
Reference
Bouchez D., Tokuhisa J.G., Llewellyn D.J., Dennis E.S. and Ellis J.G. (1989) The ocselement is a component of the promoters of several T-DNA and plant viral genes. EMBO J. 8: 4197–4204.
Escher A., O’Kane D.J., Lee J. and Szalay A.A. (1989) Bacterial luciferase aß fusion protein is fully active as a monomer and highly sensitive in vivo to elevated temperature. Proc. Natl. Acad. Sci. USA 86: 6528–6532.
Karcher S.J., DiRita V.J. and Gelvin S.B. (1984) Transcript analysis of TR DNA in octopinetype crown gall tumors. Mol. Gen. Genet. 194: 159–165.
Koncz C., Olsson O., Langridge W.H.R., Schell J. and Szalay A.A. (1987). Expression and assembly of functional bacterial luciferase in plants. Proc. Natl. Acad. Sci. USA 84: 131–135.
Langridge W.H.R., Fitzgerald K.J., Koncz C., Schell J. and Szalay A.A. (1989) Dual promoter of Agrobacterium tumefaciens mannopine synthase genes is regulated by plant growth hormones. Proc. Natl. Acad. Sci. USA 86: 3219–3223.
Maas C. and Wen W. (1989) Mechanism and optimized conditions for PEG mediated DNA transfection into plant protoplasts. Plant Cell Rep. 8: 148–151.
Olsson O., Koncz C. and Szalay A.A. (1988) The use of the luxA gene of the bacterial luciferase operon as a reporter gene. Mol. Gen. Genet. 215: 1–9.
Teeri T.H., Lehväslaiho H., Franck M., Uotila J., Heino P., Palva E.T., Van Montagu M. and Herrera-Estrella L. (1989) Gene fusions to lacZ reveal new expression patterns of chimeric genes in transgenic plants. EMBO J. 8: 343–350.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1992 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Båga, M., Langridge, W.H.R., Szalay, A.A. (1992). A functional analysis of the plant hormone-regulated dual mannopine synthase (mas) promoter region. In: Karssen, C.M., van Loon, L.C., Vreugdenhil, D. (eds) Progress in Plant Growth Regulation. Current Plant Science and Biotechnology in Agriculture, vol 13. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2458-4_93
Download citation
DOI: https://doi.org/10.1007/978-94-011-2458-4_93
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-5084-5
Online ISBN: 978-94-011-2458-4
eBook Packages: Springer Book Archive