Animal Cell Technology: Basic & Applied Aspects pp 335-340 | Cite as
Towards a Strategy for High Density Cultures of Vero Cells in Stirred Tank Reactors
Abstract
Results from batch and continuous perfusion cultivations of Vero-cells are presented. Using standard medium, highest cell densities were reached by continuous perfusion, whereas cell yield per unit of medium was highest in batch cultivation. Amino acid consumption-and production patterns were in both systems comparable, except for alanine. Alanine was produced during continuous perfusion and slightly consumed during batch cultivation. Results from measurement and control equipment can be used to calculate cell density on-line. Image analysis can be employed to analyse cell distribution over microcarriers.
Keywords
Batch Cultivation Vero Cell Cell Yield Stir Tank Reactor Medium UtilizationPreview
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