Isolation of Pure Populations of Insect Haemocytes

  • N. A. Ratcliffe
Part of the Series Entomologica book series (SENT, volume 48)

Abstract

Most living systems consist of mixed cell populations which interact in complex ways to maintain the functional integrity of the whole organism. Such interactions are particularly well illustrated in the vertebrate immune system in which, for example, macrophages and T- and B-lymphocytes cooperate intimately to mediate the cellular and humoral immune defences.

Keywords

Granular Cell Spherule Cell Percoll Gradient Continuous Gradient Discontinuous Gradient 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Ali, F.M.K. 1986. Separation of Human Blood and Bone Marrow Cells. IOP Publishing Ltd., Bristol.Google Scholar
  2. Anggraeni, T. and N.A. Ratcliffe. 1991. Studies on cell-cell cooperation during phagocytosis by purified haemocyte populations of the wax moth, Galleria mellonèlla. J. Insect Physiol. 37: 453–460.CrossRefGoogle Scholar
  3. Bohn, H. 1977. Differential adhesion of the haemocytes of Leucophaea maderae (Blattaria) to a glass surface. /. Insect Physiol. 23: 185–194.CrossRefGoogle Scholar
  4. Brillouet, C, M. Leclerc, R.A. Binaghi and G. Luguet. 1984. Specific immune response in the sea star Asterias rubens: Production of ‘antibody like’ factors. Cell Immunol. 84: 138–144.PubMedCrossRefGoogle Scholar
  5. Chain, B.M. and R.S. Anderson. 1982. Selective depletion of the plasmatocytes in Galleria mellonella following injection of bacteria. J. Insect Physiol 28: 377–384.CrossRefGoogle Scholar
  6. Cook, D., D.B. Stoltz and C. Pauley. 1985. Purification and preliminary characterization of insect spherulocytes. Insect Biochem. 15: 419–426.CrossRefGoogle Scholar
  7. Frakas, R. 1991. Separation of plasmatocytes and granulocytes of the greater wax moth, Galleria mellonella, by centrifugation on Ficoll-Paque, and patterns of protein synthesis. Dev. Comp. Immunol, (in press).Google Scholar
  8. Gupta, S. and R.A. Good. 1980. Markers of human lymphocyte subpopulations in primary immunodeficiency and lymphoproliferative disorders. Sem. Hematol. 17: 1–29.Google Scholar
  9. Huxham, I.M. and A.M. Lackie. 1988. Behaviour in vitro of separated haemocytes from the locust, Schistocerca gregaria. Cell Tissue Res. 251: 677–684.CrossRefGoogle Scholar
  10. Johansson, M.W. and K. Söderhall. 1989. Cellular immunity in crustaceans and the propo system. Parasitology Today. 5: 183–190.CrossRefGoogle Scholar
  11. Kobayashi, M., M.W. Johansson and K. Söderhall. 1990. The 76kD cell-adhesion factor from crayfish haemocytes promotes encapsulation in vitro. Cell Tissue Res. 260: 13–18.CrossRefGoogle Scholar
  12. Leonard, C.M., K. Söderhall and N.A. Ratcliffe. 1985. Studies on prophenoloxidase and protease activity of Blaberus craniifer haemocytes. Insect Biochem. 15: 803–810.CrossRefGoogle Scholar
  13. Mead, G.P., N.A. Ratcliffe and L.R. Renwrantz. 1986. The separation of insect haemocyte types on Percoll gradients: Methodology and problems. J. Insect Physiol 32: 167–177.CrossRefGoogle Scholar
  14. Peake, P.W. 1979. Isolation and characterization of the haemocytes of Calliphora vicina on density gradients of Ficoll. ]. Insect Physiol 25: 795–803.CrossRefGoogle Scholar
  15. Price, C.D. and N.A. Ratcliffe. 1974. A reappraisal of insect haemocyte classification by the examination of blood from fifteen insect orders. Z. Zellforsch. Mikrosk. Anat. 147: 537–549.CrossRefGoogle Scholar
  16. Ratcliffe, N.A., CM. Leonard and A.F. Rowley. 1984. Prophenoloxidase activation: Non-self recognition and cell cooperation in insect immunity. Science 226: 557–559.PubMedCrossRefGoogle Scholar
  17. Ratcliffe, N.A., G.P. Mead and L.R. Renwrantz. 1986. Insect haemocyte separation—an essential prerequisite to progress in understanding insect cellular immunity. In: Immunity in Invertebrates, pp. 3–11 ( M. Brehelin, ed.). Springer-Verlag, Berlin.CrossRefGoogle Scholar
  18. Ratcliffe, N.A., J.L. Broohman and A.F. Rowley. 1991. Activation of the prophenoloxidase cascade and initiation of nodule formation in locusts by bacterial lipopolysaccharides. Dev. Comp. Immunol. 15: 33–39.PubMedCrossRefGoogle Scholar
  19. Ratcliffe, N.A. and S.J. Gagen. 1977. Studies on the in vivo cellular reactions of insects: An ultrastructural analysis of nodule formation in Galleria mellonella. Tissue Cell 9: 73–85.CrossRefGoogle Scholar
  20. Söderhall, K. 1981. Fungal cell wall p 1,3-glucans induce clotting and phenoloxidase attachment to foreign surfaces of crayfish haemocyte lysate. Dev. Comp. Immunol. 5: 565–575.PubMedGoogle Scholar
  21. Söderhall, K. and V.J. Smith. 1983. Separation of the haemocyte populations of Carcinus maenas and other marine decapods, and prophenoloxidase distribution. Dev. Comp. Immunol 7: 229–239.PubMedCrossRefGoogle Scholar
  22. Söderhall, K. and V.J. Smith. 1986. Prophenoloxidase-activating cascade as a recognition and defense system in arthropods. In: Hemocytic and Humoral Immunity in Arthropods, pp. 251–285 ( A. Gupta, ed.). John Willey and Sons, New York.Google Scholar
  23. Söderhall, K., V.J. Smith and M.W. Johansson. 1986. Exocytosis and uptake of bacteria by isolated haemocyte population of two crustaceans: Evidence for cellular cooperation in the defence reaction of arthropods. Cell Tissue Res. 245: 43–49.CrossRefGoogle Scholar

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© Springer Science+Business Media Dordrecht 1993

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  • N. A. Ratcliffe

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