Regulation of the iron transport genes encoded by the pJM1 virulence plasmid in Vibrio anguillarum

  • Jorge H. Crosa
  • Luis A. Actis
  • Patricia Salinas
  • Marcelo E. Tolmasky
  • Lillian S. Waldbeser
Chapter
Part of the Developments in Plant Pathology book series (DIPP, volume 3)

Abstract

An important component of the virulence of Vibrio anguillarum 775 is the pJMl plasmid-mediated iron uptake system which consists of the siderophore anguibactin and the iron transport proteins FatA-D. Expression of this system is repressed when the iron concentration of the cell is high. We have now shown that under iron-rich conditions two elements may a play a role in the control of the expression of the fat A gene: a Fur-like product and a 600 nucleotides antisense RNA, RNAα, which is encoded in the fatA-fatB intergenic region and is induced at high iron concentrations. Under iron-limiting conditions two positive regulators, Taf and AngR, are necessary for full expression of the siderophore biosynthetic genes. AngR, a 110 kDa protein, possesses two helix-turn-helix domains with adjacent leucine zippers that may play a role in the regulatory activity of this protein as assessed by site-directed mutagenesis. We have also identified a novel catalytic activity of AngR suggesting that, in addition to its regulatory role, it acts as an enzyme in the biosynthesis of anguibactin.

Keywords

Leucine Zipper Iron Transport Vibrio Anguillarum Iron Uptake System Total Membrane Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

AMP

Adenosme Monophoshate

EDDA

Ethylenediamine-di Co-hydroxyphenyla-cetic Acid

MIC

Minimal Inhibitory Concentration

SDS-page

Sodium Dodecyl Salphate-Poly Acrylamide Gel Electrophorhesis

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Copyright information

© Springer Science+Business Media Dordrecht 1994

Authors and Affiliations

  • Jorge H. Crosa
  • Luis A. Actis
  • Patricia Salinas
  • Marcelo E. Tolmasky
  • Lillian S. Waldbeser

There are no affiliations available

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