Progress in Temperate Fruit Breeding pp 321-325 | Cite as
The use of isoenzyme polymorphisms as an aid for cultivar identification in strawberry
Summary
The study was intended to uniquely classify 32 strawberry cultivars and two breeders’ selections, mostly of Northern European origin, using four polymorphic enzyme systems: phosphoglucoisomerase (PGI), phosphoglucomutase (PGM), esterase (EST) and leucine amino peptidase (LAP). Three of these (PGI, PGM and LAP) had been used previously for this purpose by workers in North America. The techniques of both starch (SGE) and polyacrylamide gel electrophoresis (PAGE) were used. Consistent banding patterns were obtained for PGI, PGM and LAP while EST produced a large number of sharp bands but the patterns were inconsistent. The three stable enzymes only were thus used for cultivar characterisation. Banding patterns were divided phenotypically to produce three regions for PGI and two for PGM and LAP Nine different banding categories were observed for PGI, 13 for PGM and 10 for LAP. In combination these three enzyme systems permitted 30 of the 34 clones examined to be uniquely characterised. Although much of the material is closely related, e.g. nine of the cultivars have Gorella as one parent, the technique offers a high level of discrimination. In the two cases where it was not possible to separate cultivars no common ancestors for at least three generations exist. The procedure will assist greatly in cases of misidentification where a particular clone is known to be one of a small number of alternatives. Also, the banding patterns could be used as an additional criterion for identification, alongside various morphological characteristics, when publishing cultivar descriptions.
Key words
Cultivar identification isoenzymes strawberry fragaria × ananassaPreview
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