Summary
A new and easy to handle reusable minifermenter for high-density culture of hybridoma cells and other cells is described. In the case of hybridoma cells cell densities of more than 10 million cells per ml, monoclonal antibody concentrations in the range of 0.5 - 4.5 mg/ml and antibody yields in the range of 20 - 100 mg were obtained within one to four weeks.
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Köhler,G. and Milstein,C. (1975) Continuous culture of fused cells secreting antibody with predefined specifity, Nature 256, 495–497.
Falkenberg,F.W., Weichert,H., Krane,M., Bartels,I., Nagels,H.-O. and Fiebig,H. (1994) In vitro production of monoclonal antibodies in high concentration in a new and easy to handle modular minifermenter (submitted)
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© 1995 Springer Science+Business Media Dordrecht
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Weichert, H., Falkenberg, F.W., Krane, M., Behn, I., Hommel, U., Nagels, H.O. (1995). Cultivation of Animal Cells in a New Modular Minifermenter. In: Beuvery, E.C., Griffiths, J.B., Zeijlemaker, W.P. (eds) Animal Cell Technology: Developments Towards the 21st Century. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0437-1_145
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DOI: https://doi.org/10.1007/978-94-011-0437-1_145
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-4195-9
Online ISBN: 978-94-011-0437-1
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