Determination of Ks-Values (Monod Constants) for Essential Amino Acids Using CHO and Hybridoma Cells in Batch and Chemostat Cultures
Chapter
Abstract
Based on batch and chemostat cultures the Ksvalues for essential amino acids were determined and calculated using Monod equations and their simulation on computer. Results of a recombinant CHO cell line, producing human tissue plasminogen activator (t-PA) and a mouse-mouse hybridoma cell line are presented. The data of these experiments were compared with the results of the computer simulation in order to prove or dismiss the preceding model. Most of the Ksvalues were found at 10 pmol L-1 or below. Best specific productivity was determined at low dilution rates, best volumetric productivity at half maximal growth rates during chemostat cultures.
Keywords
Dilution Rate Essential Amino Acid Amino Acid Concentration Chemostat Culture Chemostat Experiment
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© Springer Science+Business Media Dordrecht 1995