Abstract
Some tumor cells become resistant to structurally unrelated antitumor drugs. The multidrug resistance phenotype (MDR) is characterized by a lowering of drug cellular accumulation associated with the expression of a 170-kDa glycoprotein, Pgp (Permeability glycoprotein) at the membrane level [for review see: Gottesman and Pastan, 1993; Simon and Schindler, 1994].
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Cossarizza, A., Baccarani-Contri, M., Kalashnikova, G., and Franceschi, C., (1993) A new method for cytofluorimetric analysis of mitochondrial membrane potential using the J-aggregate forming lipophilic cation 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolcarbocyanine iodide (JC-1). Biochem. Biophys. Res. Com. 197,40–45.
Ford, M.J., and Hait W.N., (1990) Pharmacology of drugs that alter multidrug resistance in cancer. Pharmacol. Rev. 42, 155–199.
Gottesman, M.M., and Pastan, I., (1993) Biochemistry of multidrug resistance mediated by the multidrug transporter. Ann. Rev. Biochem. 62, 385–427.
Gros, P., Talbot, F., Tang-Wai, D. Bibi, E. and Kaback, HR., (1992) Lipophilic cations: a group of model substrates for the multidrug resistance transporter. Biochemistry 31, 1992–1998.
Kessel, D., Beck, W.T., Kukuruga, D., and Schulz, V., (1991) Characterisation of multidrug resistance by fluorescent dyes. Cancer Res. 51, 4665–4670.
Maynadié, M, Lizar, G., Solary, E., Parmeland, C., et Carli, P.M., (1994) Intérêt de la rhodamine 123 pour la détection de faible proportions de cellules multichimiorésistantes. Bull. Cancer 81, 409–413.
Poupon, M.F., Marie, J.P, et Chevillard, S., (1994), Rapport du symposium international: “Resistance against anticancer drugs: molecular mechanisms and clinical opportunities”. Bull. Cancer 81, 145–156.
Ramu, A., Pollard, H.B. and Rosario, L.M., (1989) Doxorubicin resistance in P388 leukaemia- Evidence for reduced drug influx. Int. J. Cancer, 44, 539–547.
Reers, M., Smith, T.W., and Chen, L.B., (1991) J-Aggregate formation of a carbocyanine as a quantitative fluorescent indicator of membrane potential. Biochemistry 30, 4480–4486.
Simon, S.M., and Schindler, M., (1994) Cell biological mechanisms of multidrug resistance in tumors. Proc. Natl. Acad. Sci. USA 91, 3497–3504.
Smiley, S.T., Reers, M., Mattola-Hartshorn, C., Lin, M., Chen, A., Smith, T.W., Steele, G.D., and Chen, L.B., (1991) Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate forming lipophilic cation JC-1. Proc. Natl. Acad. Sci. USA, 88, 3671–3675.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1995 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Kühnel, J., Ferte, J., Sureau, F., Schwaller, M. (1995). Spectrofluorimetric Investigation of Multidrug Resistant Versus Sensitive Cells Using JC-1. In: Merlin, J.C., Turrell, S., Huvenne, J.P. (eds) Spectroscopy of Biological Molecules. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0371-8_243
Download citation
DOI: https://doi.org/10.1007/978-94-011-0371-8_243
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-4166-9
Online ISBN: 978-94-011-0371-8
eBook Packages: Springer Book Archive