Cell Culture Engineering IV pp 111-116 | Cite as
Low temperature cultivation — A step towards process optimisation
Chapter
Abstract
Adherent recombinant BHK cells were cultivated at temperatures between 30 and 37°C. Batch and repeated-batch-cultivations in a 2-litre bioreactor showed a significant influence on metabolism and cell growth. The low-temperature-cultivations showed a lower growth rate and a lower glucose consumption rate and, therefore, less lactate production. On the other hand, the maximum cell density and productivity seemed not to be affected by the temperature reduction.
Key words
Adherent animal cells glucose lactate productivity temperaturePreview
Unable to display preview. Download preview PDF.
References
- Bloemkolk J-W, Gray MR, Merchant F and Mosmann TR (1992) Effect of temperature on hybridoma cell cycle and Mab production. Biotechnol. and Bioeng. 40: 427–431.CrossRefGoogle Scholar
- Ludwig A, Tomeczkowski J and Kretzmer G (1992) Influence of the temperature on the shear stress sensibility of adherent BHK 21 cells. Appl. Microbiol. Biotechnol. 38: 323–327.PubMedCrossRefGoogle Scholar
- Reuveny S, Velez D, Macmillan JD and Miller L (1986) Factors affecting cell growth and monoclonal antibody production in stirred reactors. J. Immunol. Methods 86: 53–59.PubMedCrossRefGoogle Scholar
- Sureshkumar GK and Mutharasan R (1991) The influence of temperature on a mouse mouse hybridoma growth and monoclonal antibody production. Biotechnol. and Bioeng. 37: 292–295.CrossRefGoogle Scholar
- Tomeczkowski J, Ludwig A and Kretzmer G (1993) Effect of cholesterol addition on growth kinetics and shear stress sensitivity of mammalian cells. Enzyme Microbial Technol. 15: 849–853.CrossRefGoogle Scholar
Copyright information
© Springer Science+Business Media Dordrecht 1994