Advertisement

The freshwater planarian Dugesia (G.) tigrina contains a great diversity of homeobox genes

  • Emili Saló
  • Ana Maria Muñoz-Mármol
  • José Ramon Bayascas-Ramirez
  • Jordi Garcia-Fernàndez
  • Agusti Miralles
  • Andreu Casali
  • Montserrat Corominas
  • Jaume Baguñá
Conference paper
Part of the Developments in Hydrobiology book series (DIHY, volume 108)

Abstract

To identify potential pattern control and cell determination and/or differentiation genes in the freshwater planarian Dugesial (G.) tigrina, we searched for homeobox genes of different types in the genome of this primitive metazoan. We applied two basic approaches: 1) Screening the cDNA library with degenerate oligonucleotides corresponding to the most conserved amino acid sequence from helix-3 of the homeodomain of each family; and 2) PCR amplification of genomic DNA or cDNA, using two sets of degenerated oligonucleotides corresponding to helices 1 and 3 of the homeodomain or two specific domains of the POU family. Using the first strategy we have identified and characterized two tissue-specific cell determination and/or differentiation NK-type homeobox genes. Using the second strategy we have identified several homeobox genes that belong to the HOM/Hox, paired (prd) or POU families.

Key words

Turbellaria Dugesia homeobox gene expression 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Addoutte, A. & H. Philippe, 1993. The major lines of metazoan evolution: Summary of traditional evidence and lessons from ribosomal RNA sequence analysis. In. Y. Pichon (ed.), Comparative Molecular Neurobiology. Birhauser Verlag. Basel: Switzerland: 1–34.Google Scholar
  2. Baguñá, J., R. Romero, E. Saló, J. Collet, C. Auladell, M. Ribas, M. Riutort, J. Garcia-Fernàndez, F. Burgaya & D. Bueno, 1990. Growth, degrowth and regenerations as developmental phenomena in adult freshwater planarians. In: Experimental Embryology in Aquatic Plants and Animals. (ed. H. J. Marthy.): New York: Plenum Press. pp.: 129–162.CrossRefGoogle Scholar
  3. Bartels, J. L., M. T. Murtha, & F. H. Ruddle, 1993. Multiple Hox/HOM-class homeoboxes in Platyhelminthes. Mol. Phyl. and Evol. 2 No 2: 143–151.CrossRefGoogle Scholar
  4. Billin, A. N., K. A. Cockerill, & S. J. Poole (1991). Isolation of a family of Prosophila POU domain genes expressed in early development. Mech Dev. 34: 75–84.PubMedCrossRefGoogle Scholar
  5. Bürglin, T. R., M. Finny, A. Coulson, & G. Ruvkun, 1989.Caenorhabditis elegar s has scores of homeobox-containing genes. Nature 341: 239–243.PubMedCrossRefGoogle Scholar
  6. Bürglin, T. R. 1993. The homeodomain phylum. In A guidebook for homeobox genes. D. Duboule (ed.). Oxford: Oxford University Press. In press.Google Scholar
  7. Forhman, M. A., M. Boyle, & G. R. Martin, 1990. Isolation of the mouse Hox-2.9 gene; analysis of embryonic expression suggests that positional information along the anterior-posterior axis is specified by mesoderm. Development 110: 589–607.Google Scholar
  8. Garcia-Fernàndez, J., J. Baguñá, & E. Saló, 1991. Planarian homeobox genes: Cloning, sequence analysis, and expression. Proc. Natl. Acad. Sci. USA 88: 7338–7342.PubMedCrossRefGoogle Scholar
  9. Garcia-Fernàndez, J., J. Baguñá, & E. Saló, 1993. Genomic organization and expression of the planarian homeobox genes Dth-1 and Dth-2. Development 11 8: 241–253.Google Scholar
  10. Gehring, W. J. 1987. Homeoboxes in the study of development. Science 236: 1245–1252.PubMedCrossRefGoogle Scholar
  11. He, X., M. N. Treacy, D. M. Simmons, H. A. Ingraham, L. W. Swanson, & M. G. Rosenfeld, 1989 Nature 340: 35–42.Google Scholar
  12. Holland, P. W. H., 1992. Homeobox genes in vertebrate evolution. Bio Essays 14: 267–273.Google Scholar
  13. McGinnis, W. & Krumlauf, R. 1992. Homeobox genes and axial patterning. Cell 68: 283–302PubMedCrossRefGoogle Scholar
  14. Miller, D. J. & A. Miles, 1993. Homeobox genes and the zootype. Nature 365: 215–216.PubMedCrossRefGoogle Scholar
  15. Orii, H., K. Agata, & K. Watanabe, 1993. POU-domain genes in planarian Dugesia japonica: The structure and expression. Biochem. Bioph. Res. Commun. 192: 1395–1402.Google Scholar
  16. Riutort, M., K. G. Field, J. M. Turbeville, R. A. Raff, & J. Baguñá 1992. Enzyme electrophoresis, 18S rRNA sequences, and levels of phylogenetic resolution among several species of freshwater planarians (Platyhelminthes, Tricladida, Paludicola). Can. J. Zool., 70: 1425–1439.CrossRefGoogle Scholar
  17. Saló, E. 1984. Formació del blastema i re-especificació del patró durant la regeneració de les planàries Dugesia (S) mediterranea i Dugesia (G.) tigrina: anàlisi morfologica, cel.lular i bioquimica, Ph. D. Thesis, University of Barcelona.Google Scholar
  18. Saló, E. & J. Baguñá, 1984. Regeneration and pattern formation in planarians. I. The pattern of mitosis in anterior and posterior regeneration in Dugesia (G) tigrina, and a new proposal for blastema formation. J. Embryol. Exp. Morph. 83: 63–80.Google Scholar
  19. Saló, E. & J. Baguñá, 1989. Regeneration and pattern formation in planarians. II. Local origin and role of cell movements in blastema formation. Development 107: 69–76.Google Scholar
  20. Schubert, F. R., K. Nieselt-Struwe & P. Gruss, 1993. The Antennapediatype homeobox genes have evolved from three precursors separated early in metazoan evolution. Proc. Natl. Acad. Sci. USA. 90: 143–147.PubMedCrossRefGoogle Scholar
  21. Shenk, M. A., H. Bode & R. E. Steele, 1993. Expression of Cnox-2, a HOM/HOX homeobox gene in hydra, is correlated with axial pattern formation. Development 117: 657–667.PubMedGoogle Scholar
  22. Slack, J. M. W., P. W. H. Holland & C. F. Graham, 1993. The zootype and the phylotypic stage. Nature 361: 490–492.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 1995

Authors and Affiliations

  • Emili Saló
    • 1
  • Ana Maria Muñoz-Mármol
    • 1
  • José Ramon Bayascas-Ramirez
    • 1
  • Jordi Garcia-Fernàndez
    • 1
  • Agusti Miralles
    • 1
  • Andreu Casali
    • 1
  • Montserrat Corominas
    • 1
  • Jaume Baguñá
    • 1
  1. 1.Departament de Genètica, Facultat de BiologiaUniversitat de BarcelonaBarcelonaSpain

Personalised recommendations