Genetic Transformation of Plants by Agrobacterium

  • Donald Grierson
  • Simon N. Covey
Part of the Tertiary Level Biology book series (TLB)


Agrobacterium tumefaciens causes crown gall disease in plants. Crown galls are cancerous growths composed of disorganized proliferating plant cells, often found near soil level at the junction of plant stem and root. Agrobacterium is an oncogenic agent which genetically transforms plant cells and directs the resulting tumours to synthesize special nutrients that support growth of the bacteria. A competitive advantage is conferred upon the inciting Agrobacterium strain because only it, or a related strain, can utilize the nutrients produced by the tumour as an energy source. The transformation process involves transfer of genetic information from the bacteria to the plant stimulated by compounds exuded from wounded plant cells. This is the only known example of inter-kingdom exchange of genetic material and has been described as ‘genetic colonization’ (Schell et al., 1979).


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  1. Barker, R.F., Idler, K.B., Thompson, D.V. and Kemp, J.D. (1983) Nucleotide sequence of the T-DNA region of Agrobacterium tumefaciens octopine Ti plasmid pTi 15955. Plant. Mol. Biol. 2, 335–350.CrossRefPubMedGoogle Scholar
  2. Braun, A.C. (1982) A history of the crown gall problem. In Molecular Biology of Plant Tumours, eds. G. Kahl and J. Schell, Academic Press, New York, 155–210.Google Scholar
  3. Buchmann, I., Marner, F-J., Schröder, G., Waffenschmidt, S. and Schröder, J. (1985) Tumour genes in plants: T-DNA encoded cytokinin biosynthesis EMBO J. 4, 853–859.PubMedPubMedCentralCrossRefGoogle Scholar
  4. Bytebier, B., Deboeck, F., De Greve, H., Van Montagu, M. and Hernalsteens, J-P. (1987) T-DNA organization in tumour cultures and transgenic plants of the monocotyledon Asparagus officinalis. Proc. Natl. Acad. Sci. USA 84, 5345–5349.CrossRefPubMedGoogle Scholar
  5. Caplan, A., Herrera-Estrella, L., Inze, D., Van Haute, E., Van Montagu, M., Schell, J. and Zambryski, P. (1983) Introduction of genetic material into plant cells. Science 222, 815–821.CrossRefPubMedGoogle Scholar
  6. Chilton, M-D., Drummond, M.H., Merlo, D.J., Sciaky, D., Montoya, A.L., Gordon, M.P. and Nester, E.W. (1977) Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis. Cell 11, 263–271.CrossRefPubMedGoogle Scholar
  7. Depicker, A., De Wild, M., De Vos, M.G., Van Montagu, M., and Schell, J. (1980) Molecular cloning of the nopaline Ti plasmid pTiC58 and its use for restriction endonuclease mapping. Plasmid 3, 193–211.CrossRefPubMedGoogle Scholar
  8. De Vos, G., De Beuckeleer, M., Van Montagu, M. and Schell, J. (1981) Restriction endonuclease mapping of the octopine tumour-inducing plasmid pTiAch5 of Agrobacterium tumefaciens. Plasmid 6, 249–253.CrossRefPubMedGoogle Scholar
  9. Gheysen, G., Van Montagu, M. and Zambryski, P. (1987) Integration of Agrobacterium tumefaciens transfer DNA (T-DNA) involves rearrangements of target plant DNA sequences. Proc. Natl. Acad. Sci. USA 84, 6169–6173.CrossRefPubMedGoogle Scholar
  10. Hamilton, R.H. and Fall, M.Z. (1971) The loss of tumour-initiating ability in Agrobacterium tumefaciens by incubation at high temperature. Experientia 27, 229–230.CrossRefPubMedGoogle Scholar
  11. Hooykaas, P.J.J. and Schilperoort, R.A. (1985) The Ti plasmid of Agrobacterium tumefaciens: a natural genetic engineer. Trends Biochem. Sci. 10, 307–309.CrossRefGoogle Scholar
  12. Kerr, A. (1969) Transfer of virulence between strains of Agrobacterium. Nature (London) 223, 1175–1176.CrossRefGoogle Scholar
  13. Koukolikovâ-Nicola, Z., Shillito, R.D., Hohn, B., Wang, K., Van Montagu, M. and Schell, J. (1985) Involvement of circular intermediates in the transfer of T-DNA from Agrobacterium tumefaciens to plant cells. Nature (London) 313, 191–196.CrossRefGoogle Scholar
  14. Koukolikovâ-Nicola, Z., Albright, L. and Hohn, B. (1987) The mechanism of T-DNA transfer from Agrobacterium tumefaciens to the plant cell. In Plant DNA Infectious Agents, eds. Th. Hohn and J. Schell, Springer Verlag, Wien, New York, 109–48.CrossRefGoogle Scholar
  15. Lichtenstein, C. (1986) A bizarre vegetal bestiality. Nature (London) 322, 682–683.CrossRefGoogle Scholar
  16. Nester, E.W., Gordon, M.P., Amasino, R.M. and Yanofsky, M.F. (1984) Crown gall: a molecular and physiological analysis. Ann. Rev. Plant Physiol. 35, 387–413.CrossRefGoogle Scholar
  17. Petit, A. and Tempé, J. (1975) The function of T-DNA in nature. In Molecular Form and Function of the Plant Genome, ed. L. van Vloten-Doting, Plenum Press, New York, 625–636.Google Scholar
  18. Schell, J., Van Montagu, M., De Beuckeleer, M., De Block, M., Depicker, A., De Wilde, M., Engler, G., Gentello, C., Hernalsteens, J.P., Holsters, M., Seurinck, J., Silver, B., Van Vliet, F. and Villarroel, R. (1979) Interactions and DNA transfer between Agrobacterium tumefaciens, the Ti plasmid and the host. Proc. R. Soc. London. Ser. B, 204, 251–266.CrossRefGoogle Scholar
  19. Stachel, S.E., Messens, E., Van Montagu, M. and Zambryski, P. (1985) Identification of the signal molecules produced by wounded plant cells that activate T-DNA transfer in Agrobacterium tumefaciens. Nature (London) 318, 624–629.CrossRefGoogle Scholar
  20. Stachel, S.E. and Zambryski, P. (1986) Agrobacterium tumefaciens and the susceptible plant cell: a novel adaptation of extracellular recognition and DNA conjugation. Cell 47, 155–157.PubMedGoogle Scholar
  21. Stachel, S.E., Timmerman, B. and Zambryski, P. (1986) Generation of single-stranded T-DNA molecules during the initial stages of T-DNA transfer from Agrobacterium tumefaciens to plant cells. Nature (London) 322, 706–712.CrossRefGoogle Scholar
  22. Tepfer, M. and Casse-Delbart, F. (1987) Agrobacterium rhizogenes as a vector for transforming higher plants. Microbiol. Sci. 4, 24–28.PubMedGoogle Scholar
  23. Thomashow, M.F., Hugly, S., Buchholz, W.G. and Thomashow, L.S. (1986) Molecular basis for the auxin-independent phenotype of crown gall tumour tissues. Science 231, 616–618.CrossRefPubMedGoogle Scholar
  24. Usami, S., Morikawa, S., Takebe, I. and Machida, Y. (1987) Absence in monocotyledenous plants of the diffusible plant factors inducing T-DNA circularization and vir gene expression in Agrobacterium. Mol. Gen. Genet. 209, 221–226.CrossRefPubMedGoogle Scholar
  25. Van Larabeke, N., Gentello, C., Schell, J., Schilperoort, R.A., Hermans, A.K., Hernalsteens, J.P. and Van Montagu, M. (1975) Acquisition of tumour-inducing ability by non-oncogenic agrobacteria as a result of plasmid transfer. Nature (London) 255, 742–743.CrossRefGoogle Scholar
  26. Watson, B., Currier, T.C., Gordon, M.D., Chilton, M-D. and Nester, E.W. (1975) Plasmid required for virulence of Agrobacterium tumefaciens. J. Bacteriol. 123, 255–264.PubMedGoogle Scholar
  27. Weiler, E.W. and Schröder, J. (1987) Hormone genes and crown gall disease. Trends Biochem. Sci. 12, 271–275.CrossRefGoogle Scholar
  28. Yadev, N.S., Vanderleyden, J., Bennett, D.R., Barnes, W.M. and Chilton, M-D. (1982) Short direct repeats flank the T-DNA on the nopaline Ti plasmid. Proc. Natl. Acad. Sci. USA 79, 6322–6326.CrossRefGoogle Scholar
  29. Zaenen, I., van Larebeke, N., Teuchy, N., van Montagu, M. and Schell, J. (1974) Supercoiled circular DNA in crown gall inducing Agrobacterium strains. J. Mol. Biol. 86, 109–127.CrossRefPubMedGoogle Scholar
  30. Zambryski, P., Depicker, A., Kriger, K. and Goodman, H. (1982) Tumour induction by Agrobacterium tumefaciens: analysis of the boundaries of T-DNA. J. Mol. Appl. Genet. 1, 361–370.PubMedGoogle Scholar

Copyright information

© Chapman & Hall 1988

Authors and Affiliations

  • Donald Grierson
    • 1
  • Simon N. Covey
    • 2
  1. 1.Department of Physiology and Environmental ScienceUniversity of NottinghamUK
  2. 2.John Innes Institute, AFRC Institute of Plant Science ResearchNorwichUK

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