Regulatory mediators in Arthus reactions: lysosomal enzymes in biopsy and serum
The accumulation of polymorphonuclear leukocytes (PMN) has been demonstrated as a central pathogenic event in inflammatory lesions like the Arthus reaction1. During the last decade, the possible role of these cells in acute inflammatory processes has been studied extensively in vitro. As a main focus of these investigations it has become clear that PMN, whenever they encounter immunological stimuli like immune complexes or C5a, release their granule constituents2, 3. The various hydrolytic enzymes stored within different granules take part in the cellular bactericidal systems as well as in the digestion of ingested material (bacteria, immune complexes). However, they have also been demonstrated to degrade a wide variety of tissue substrates in vitro (for review see reference 3). Therefore, the release of proteolytic activities could be an important contribution to the injury which occurs in the inflamed tissue in vivo.
KeywordsNormal Skin Neutral Protease Regulatory Mediator Elastase Activity Human Polymorphonuclear Leukocyte
Unable to display preview. Download preview PDF.
- 7.Schmidt, M.E., Douglas, S.D., Quie, P. et al. (1978). Effect of neutral granulocyte proteases on human immunocompetent cells: Action of elastase-like protease and chymotrypsin-like protease on mononuclear phagocytes. In Havemann, K. and Janoff, A. (eds.) Neutral proteases of human polymorphonuclear leukocytes, p. 298. (Baltimore-Munich: Urban and Schwarzenberg)Google Scholar
- 10.Till, G., Debatin, K. M. and Gemsa, D. (1980): Regulatory mediators in Arthus reactions: demonstration of chemotactic factor inactivator and cell directed inhibitor activity. This volume, Chap. 90Google Scholar
- 13.Pütter, J. (1974). Peroxidasen. In Bergmeyer, H. U. (ed.) Methoden der enzymatischen Analyse, p. 725. (Weinheim: Verlag Chemie).Google Scholar
- 14.Fishman, W. H. (1974). β-glucuronidase. In Bergmeyer, H. U. (ed.) Methoden der enzymatischen Analyse, p. 964. (Weinheim: Verlag Chemie)Google Scholar
- 18.Ohlsson, K. (1978). Interaction of granulocyte neutral proteases with alpha,-antitrypsin, alpha2-macroglobulin and alpha,-antichymotrypsin. In Havemann, K. and Janoff, A. eds. Neutral proteases of human polymorphonuclear leukocytes, p. 167. (Baltimore-Munich: Urban & Schwarzenberg)Google Scholar