Effect of pharmacological agents on macrophage accumulation
Rheumatoid arthritis is characterized by an influx of both polymorphonuclear (PMN) and mononuclear leukocytes into various inflammatory sites1,2. These infiltrating cells are, in part, responsible for a number of pathological events resulting ultimately in protease release and connective tissue catabolism. Over the past 10 years a number of in vivo models of leukocyte infiltration have been described3–5. Most have been aimed at PMN accumulation and the effects of pharmacological agents on this process. In the current studies, a model of macrophage accumulation was developed; in addition, the effects of steroidal and non-steroidal anti-inflammatories and anti-rheumatic agents were determined.
KeywordsAcridine Orange Pleural Cavity Macrophage Accumulation Carrageenan Injection Gold Sodium Thiomalate
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- 6.Koski, I. R., Poplack, D. G., Blaese, R. M. (1976). A monospecific esterase stain for the identification of monocytes and macrophages. In Bloom, B. R. and David, J. R. (eds.) In Vitro Methods in Cell Mediated and Tumor Immunity, pp. 359–363 (New York: Academic Press)Google Scholar
- 7.Allison, A.C. (1976). Fluorescence microscopy of lymphocytes and mononuclear phagocytes and the use of silica to eliminate the latter. In Bloom, B. R. and David J. R. (eds.) In Vitro Methods in Cell Mediated and Tumor Immunity, pp. 395–404 (New York: Academic Press)Google Scholar
- 10.Ackerman, N., Tomolonis, A., Miram,L., Kheifets, J., Martinez, S. and Carter, A. (1981). Three day pleural inflammation: A new model to detect drug effects on macrophage accumulation. J. Pharm. Exp. Ther. (In press)Google Scholar
- 12.Koh, M.S., Parente, L. and Willoughby, D. A. (1979). Immunological and non-im-munological pleural inflammation: The effects of anti-inflammatory and anti-rheumatic drugs. Eur. J. Rheum. Inflamm. 1, 286Google Scholar
- 13.Ackerman, N. (Personal observations)Google Scholar