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Arachidonic acid metabolism by lipoxygenase in leukocytes of different species and the chemokinetic activity of some products (Abstract)

  • R. M. J. Palmer
  • J. A. Salmon
  • S. Narumiya
  • G. A. Higgs
  • K. E. Eakins
Part of the Inflammation: Mechanisms and Treatment book series (FTIN, volume 4)

Abstract

Mono-hydroxy acids derived from arachidonic acid (AA) via the lipoxygenase pathway have been reported to be chemokinetic and chemotatic for human leukocytes, 5-hydroxyeicosatetraenoic acid (5-HETE) being the most potent1. In the present study we have investigated the metabolism of AA in rat and rabbit peritoneal exudate cells (PEC) and in human leukocytes together with the chemokinetic activity of the products for each species. Following extraction and purification, the metabolites formed during incubation of [14C]AA with rat and rabbit PEC and human peripheral blood leukocytes were identified by UV spectrophotometry and gas chromatography mass spectrometry. Lipoxygenase activity was detected in the leukocytes of all three species but the end products varied. Normal rat PEC produced a mixture of 11- and 12-HETE while those of the rabbit formed predominantly 5-HETE and lesser amounts of 11- and 12-HETE. Human cells were also able to biosynthesize a mixture of 5-, 11- and 12-HETE. The relative chemokinetic potencies of 5-, 12- and 15-HETE and the analogous hydroperoxy acids were examined in an agarose micro-drop assay2 using rat and rabbit PEC and human peripheral leukocytes. All the acids studied were active on rabbit PEC in concentrations between 0.1 and 3.2 µg/ml with a rank order of 12-HETE > 15-HPETE > 15-HETE > AA = 5-HPETE > 5-HETE.

Keywords

Arachidonic Acid Human Leukocyte Arachidonic Acid Metabolism Lipoxygenase Activity Peritoneal Exudate Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Goetzl, E. J. and Sun, F. F. (1979). Generations of unique mono-hydroxy eicosatetraenoic acids from arachidonic acid by human neutrophils. J. Exp. Med., 150, 406PubMedCrossRefGoogle Scholar
  2. 2.
    Smith, M. J. H. and Walker, J. R. (1980). The effect of some antirheumatic drugs on an in vitro model of human polymorphonuclear leukocyte chemokinetics. Br. J. Pharmacol., 69, 433Google Scholar

Copyright information

© MTP Press Limited 1980

Authors and Affiliations

  • R. M. J. Palmer
    • 1
  • J. A. Salmon
    • 1
  • S. Narumiya
    • 1
  • G. A. Higgs
    • 1
  • K. E. Eakins
    • 1
  1. 1.UK

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