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Development of an Arthus-like model of induced inflammation and its use in the anti-inflammatory characterization of orgotein (bovine Cu-Zn superoxide dismutase)

  • L. D. Williams
  • M. G. Saifer
  • P. H.-C. Dang
  • W. Huber
Part of the Inflammation: Mechanisms and Treatment book series (FTIN, volume 4)

Abstract

The procedure of Ungar et al. 1 of gravimetrically measuring skin oedema, especially as induced by intradermal injection of anti-serum (reverse Arthus reaction), is a common model for assaying anti-inflammatory activity. We have found that the large number of subjective factors involved in the skin dissection and punching can be controlled by randomly blinding drug dosing and other parameters. However, variations in anti-serum from lot to lot may also influence the results, making it desirable to find a more uniform and less expensive inflammant.

Keywords

Intradermal Injection Average Saline Group Inflammatory Index Arthus Reaction Binding Cell Wall 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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    Ungar, G., Kobrin, S. and Sezesny, B. (1959). Measurement of inflammation and evaluation of anti-inflammatory agents. Arch. Int. Pharmacodyn. Ther., 123, 71PubMedGoogle Scholar
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    Ishizaka, K., Ishizaka, T. and Sugahara, T. (1961). Biological activities of aggregated gamma globulin. III. Production of Arthus-like reactions. J. Immunol., 86, 220PubMedGoogle Scholar
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    Cochrane, C. G. and Weigle, W.O. (1958). The cutaneous reaction to soluble antigen-antibody complexes: a comparison with the Arthus phenomenon. J. Exp. Med., 108, 591PubMedCrossRefGoogle Scholar
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    Huber, W. and Saifer, M. G. P. (1977). Orgotein the drug version of bovine Cu-Zn superoxide dismutase. I. A summary account of safety and pharmacology in laboratory animals. In Michelson, A. M., McCord, J. M. and Fridovich, I. (eds.). Superoxide and Superoxide Dismutases, 517–536. (New York: Academic Press)Google Scholar
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    Menander-Huber, K. B. (1979). Double-blind controlled clinical trials in man with bovine Cu-Zn superoxide dismutase (orgotein). In Bannister, W. H. and Bannister, J. V. (eds.) The Significance of Superoxide and Superoxide Dismutase. Vol. II. Biological and Clinical Aspects. (New York: Elsevier)Google Scholar
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    Huber, W., Menander-Huber, K. B., Saifer, M. G. P. and Williams, L. D. (1979). Bioavailability of superoxide dismutase: Implications for the anti-inflammatory action mechanism of orgotein. Agents Actions Suppl. (In press)Google Scholar

Copyright information

© MTP Press Limited 1980

Authors and Affiliations

  • L. D. Williams
    • 1
  • M. G. Saifer
    • 1
  • P. H.-C. Dang
    • 1
  • W. Huber
    • 1
  1. 1.USA

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