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A mitogenic factor for macrophages present in rat serum (Abstract)

  • N. S. Connor
  • A. W. Ford-Hutchinson
  • M. J. H. Smith
Part of the Inflammation: Mechanisms and Treatment book series (FTIN, volume 4)

Abstract

Rat macrophages, obtained from a mixed white cell population collected by peritoneal lavage, were plated out at 6 × 105 cells cm2 in Medium 199 containing 20% fetal calf serum, 25 mol/l HEPES pH 7.4 and antibiotics and were aged for 3 days in culture. The cells were then cultured for a further 6 days in fresh medium containing 20% fetal calf serum, tritiated thymidine and 0–50% normal rat serum. Mitogenesis was assessed by counting the percentage labelled nuclei following autoradiography. Colony stimulating activity was assessed using thioglycolate elicited rat peritoneal macrophages harvested 3 days after injection. The cells were seeded at 600 cells/cm2 in medium as above, in the presence or absence of rat serum or rat serum fractions and colonies were counted after 28 days incubation.

Keywords

Fetal Calf Serum Fresh Medium Immunological Response Apparent Molecular Weight Peritoneal Lavage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© MTP Press Limited 1980

Authors and Affiliations

  • N. S. Connor
    • 1
  • A. W. Ford-Hutchinson
    • 1
  • M. J. H. Smith
    • 1
  1. 1.UK

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