Quantitative measurement of human leukocyte aggregation as a possible correlation with cell mediated immunity
The study of cell mediated immunity has been facilitated by the development of in vitro methods, especially in experimental animals. However, there are no wholly satisfactory in vitro methods for use with humans. Inhibition of migrating indicator cells resulting from the interaction of sensitized lymphocytes with specific antigen and the lymphocyte transformation test (LTT) are widely used. Both of these techniques have been extensively studied and compared with in vivo delayed hypersensitivity reactions, as assessed by skin testing1–5. They both have disadvantages. The migration inhibition is cumbersome, difficult to set up and is sometimes no better than semi-quantitative. In the lymphocyte transformation test, stimulation of antibody-producing cells by antigen may also be measured. It is not surprising, therefore, that the results obtained with these tests are not always correlated with each other and with skin test results1,5–7. The direct migration inhibition of leukocytes from sensitized subjects by specific antigen is thought to be due to release of lymphognes (leukocyte inhibitory factor, LIF) from lymphocytes which inhibits the spontaneous migration of mainly polymorphonuclear leukocytes. Indirect migration inhibition can be measured by assaying culture supernatants of sensitized lymphocytes and specific antigen on the migration of normal leukocytes.
KeywordsMigration Inhibition Factor Cell Mediate Immunity Peritoneal Exudate Cell Macrophage Aggregation Migration Inhibition
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