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Characterization of 125I-Trp11-Neurotensin Binding to Rat Fundus Smooth Muscle Plasma Membranes

  • P. Kitabgi
  • C.-Y. Kwan
  • J. E. T. Fox

Abstract

The brain-gastrointestinal tridecapeptide neurotensin (NT) has been shown to affect the contractility of a variety of gastrointestinal smooth muscle preparations in vitro [1]. In particular, it has been reported that NT contracts rat fundus smooth muscle strips [2]. Rharmacological experiments and structure activity studies have suggested that this effect was myogenic in nature and involved an interaction of NT with specific smooth muscle receptors [3]. In the present paper we provide direct evidence for the existence of such receptors from radioligand binding studies using 125I-Trp11-NT as the labelled ligand and highly purified rat fundus smooth muscle plasma membranes at the source of NT receptors.

Keywords

Gastrointestinal Motility High Affinity Binding Site Radioligand Binding Study Structure Activity Study Label Ligand 
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References

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    Kwan, C.Y., Sakai, Y., Grover, A.K. and Lee, R.M. K.W. (1982). Isolation and characterization of plasma membrane fraction from gastric fundus smooth muscle of the rat. Mol. Physiol., 2, 107–120Google Scholar
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Copyright information

© MTP Press Limited 1984

Authors and Affiliations

  • P. Kitabgi
  • C.-Y. Kwan
  • J. E. T. Fox

There are no affiliations available

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